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Applying Advanced In Vitro Culturing Technology to Study the Human Gut Microbiota
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Analysis of Postmortem Intestinal Microbiota Successional Patterns with Application in Postmortem Interval

Ruina Liu1, Qi Wang2, Kai Zhang1

  • 1College of Forensic Medicine, Xi'an Jiaotong University, Xi'an, 710061, China.

Microbial Ecology
|November 14, 2021
PubMed
Summary

Understanding postmortem microbial succession is key for decomposition studies. This research reveals distinct intestinal microbial patterns after death, aiding in accurate postmortem interval estimation using microbial biomarkers.

Keywords:
16S RNAGut microbiotaMicrobial dynamic analysisPostmortem interval estimationTime-series study

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Area of Science:

  • Forensic Microbiology
  • Decomposition Ecology
  • Microbial Ecology

Background:

  • Microbial succession in vertebrate decomposition is crucial for nutrient cycling but poorly understood.
  • Postmortem microbial changes in the intestine are complex and require detailed investigation.

Purpose of the Study:

  • To analyze postmortem intestinal microbial succession patterns in a mouse model.
  • To develop a predictive model for postmortem interval estimation based on microbial data.

Main Methods:

  • Hierarchical clustering of microbial 16S rDNA sequences using Manhattan distances.
  • Development of a random forest regression model for postmortem interval prediction.

Main Results:

  • Seven distinct classes of microbial succession patterns were identified.
  • The model achieved a mean absolute error of 20.01 hours and R² of 0.95 over 15 days.
  • Lactobacillus, Dubosiella, Enterococcus, and Lachnospiraceae NK4A136 group identified as significant biomarkers.

Conclusions:

  • Postmortem intestinal flora shifts from normal to facultative and obligate anaerobes.
  • Microbial succession patterns can reliably predict postmortem intervals.
  • This study provides insights into microbial behavior during decomposition.