Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Crossing Over

Crossing Over

Unlike mitosis, meiosis aims for genetic diversity in its creation of haploid gametes. Dividing germ cells first begin this process in prophase I, where each chromosome—replicated in S phase—is now composed of two sister chromatids (identical copies) joined centrally.
The homologous pairs of sister chromosomes—one from the maternal and one from the paternal genome—then begin to align alongside each other lengthwise, matching corresponding DNA positions in a process...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Genome-wide mapping of Cas9-induced sister chromatid exchange across single and over 200 genomic targets.

bioRxiv : the preprint server for biology·2026

Same author

Lesions initiating spontaneous mitotic crossover are minimally subject to non-homologous end joining.

bioRxiv : the preprint server for biology·2026

Same author

Terabyte scale dataset for partial discharge detection in covered conductors via contact galvanic method.

Scientific data·2026

Same author

High-throughput mapping of spontaneous mitotic crossover and genome instability events with sci-L3-Strand-seq.

Nucleic acids research·2026

Same author

Association of prior history of suicide attempt and disease severity and subsequent suicidal acts among patients with psychiatric disorders in North China: a multicentre prospective study protocol.

BMJ open·2025

Same author

Exploring the top 30 drugs associated with drug-induced thrombotic microangiopathy based on the FDA adverse event reporting system.

Frontiers in pharmacology·2025

Same journal

Clinical Europium fluorescent based lectin assays for mucin O-glycomics.

Methods in enzymology·2026

Same journal

A dual-color FRET assay for detection and quantitative analysis of O-glycopeptidases.

Methods in enzymology·2026

Same journal

Evolutionary genetic approaches to analyze mucins.

Methods in enzymology·2026

Same journal

Ex vivo imaging and enzymatic analysis of intestinal mucus.

Methods in enzymology·2026

Same journal

Glyco-TRAPP: A real-time glycocalyx permeability assay for assessing transmembrane mucin barrier function in live and fixed tissues.

Methods in enzymology·2026

Same journal

Quantitative imaging approaches to capture structural and functional dynamics of colonic mucus in health and disease in situ.

Methods in enzymology·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Oct 13, 2025

Frequency and Distribution of Crossovers in Caenorhabditis elegans Meiosis by SNP Genotyping using Real-time PCR

Frequency and Distribution of Crossovers in Caenorhabditis elegans Meiosis by SNP Genotyping using Real-time PCR

Published on: July 11, 2025

A mapping platform for mitotic crossover by single-cell multi-omics.

Peter Chovanec¹, Yi Yin¹

¹Department of Human Genetics, University of California, Los Angeles, CA, Unites States.

Methods in Enzymology

|November 15, 2021

Summary

This summary is machine-generated.

We developed new high-throughput, single-cell sequencing methods to map mitotic crossovers genome-wide. These techniques can identify large-scale genome rearrangements in various eukaryotes.

Keywords:

Genome instability Homologous recombination Linear amplification Mitotic crossover Mitotic recombination Single-cell combinatorial indexing Single-cell sequencing

More Related Videos

Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility Using scRNA-Seq and scATAC-Seq

Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility Using scRNA-Seq and scATAC-Seq

Published on: March 12, 2021

Mapping Mammalian 3D Genome Interactions with Micro-C-XL

Mapping Mammalian 3D Genome Interactions with Micro-C-XL

Published on: November 3, 2023

Related Experiment Videos

Last Updated: Oct 13, 2025

Frequency and Distribution of Crossovers in Caenorhabditis elegans Meiosis by SNP Genotyping using Real-time PCR

Frequency and Distribution of Crossovers in Caenorhabditis elegans Meiosis by SNP Genotyping using Real-time PCR

Published on: July 11, 2025

Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility Using scRNA-Seq and scATAC-Seq

Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility Using scRNA-Seq and scATAC-Seq

Published on: March 12, 2021

Mapping Mammalian 3D Genome Interactions with Micro-C-XL

Mapping Mammalian 3D Genome Interactions with Micro-C-XL

Published on: November 3, 2023

Area of Science:

Genetics
Genomics
Molecular Biology

Background:

Mitotic crossovers can lead to significant genome rearrangements.
Understanding these events is crucial for genome stability research.

Purpose of the Study:

To introduce novel high-throughput, single-cell whole-genome sequencing methods.
To enable genome-wide mapping of mitotic crossovers at scale.

Main Methods:

Developed high-throughput, single-cell, whole-genome sequencing techniques.
Applied methods for genome-wide crossover mapping.

Main Results:

Successfully mapped mitotic crossovers genome-wide using the new methods.
Demonstrated the scalability of the sequencing approach.

Conclusions:

The described methods provide a powerful tool for studying genome rearrangements.
These techniques are adaptable for various eukaryotes and other genomic endpoints.