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The Naturally Evolved EPSPS From Goosegrass Confers High Glyphosate Resistance to Rice.

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Summary

Researchers developed glyphosate-resistant rice using a goosegrass 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) gene. This new system offers higher transformation efficiency and a promising alternative for developing glyphosate-resistant crops.

Keywords:
EPSPSglyphosategoosegrassplant transformationrice

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Area of Science:

  • Plant Biotechnology
  • Genetics
  • Agricultural Science

Background:

  • Glyphosate-resistant (GR) crops utilize the CP4-EPSPS gene from Agrobacterium for weed control.
  • Concerns exist regarding biosafety of microbial GR genes, often developed using hygromycin-HPT transformation.
  • Existing methods for developing GR crops have limitations.

Purpose of the Study:

  • To develop a novel glyphosate-resistant rice using a plant-derived EPSPS gene from goosegrass.
  • To investigate the integration and inheritance of the introduced gene across generations.
  • To establish and evaluate a new glyphosate selection system for enhanced plant transformation.

Main Methods:

  • Identified and isolated the 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) gene from goosegrass.
  • Developed transgenic rice expressing the goosegrass EPSPS gene (EiEPSPS).
  • Investigated transgene integration and inheritance over two generations.
  • Reconstructed a binary vector (pCEiEPSPS) for a whole-stage glyphosate selection system.

Main Results:

  • Transgenic rice (EiEPSPS) exhibited high glyphosate resistance and comparable growth to wild type without selection pressure.
  • EiEPSPS plants showed improved reproductive performance under low glyphosate selection.
  • The Glyphosate-pCEiEPSPS selection system demonstrated significantly higher transformation efficiency than the hygromycin-HPT system.

Conclusions:

  • The goosegrass EPSPS gene is a viable alternative resource for developing glyphosate-resistant plants.
  • The developed Glyphosate-pCEiEPSPS selection system enhances plant transformation efficiency.
  • This study expands the available tools for plant genetic engineering and crop improvement.