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Protein Dynamics in Living Cells

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

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Related Experiment Video

Updated: Oct 13, 2025

Characterization of Amyloid Structures in Aging C. Elegans Using Fluorescence Lifetime Imaging

Characterization of Amyloid Structures in Aging C. Elegans Using Fluorescence Lifetime Imaging

Published on: March 27, 2020

Stress response decay with aging visualized using a dual-channel logic-based fluorescent probe.

Jingye Tian¹, Donglei Shi², Yanhui Zhang¹

¹Key Laboratory of Synthetic and Natural Functional Molecule of the Ministry of Education, College of Chemistry and Materials Science, Northwest University Xi'an 710127 China guoyuan@nwu.edu.cn.

Chemical Science

|November 15, 2021

Summary

This study introduces ROKS, a novel fluorescent probe, to precisely measure stress response capacity (SRC) decay, a key aging indicator. ROKS enables better aging diagnosis and anti-aging drug efficacy evaluation.

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Last Updated: Oct 13, 2025

Characterization of Amyloid Structures in Aging C. Elegans Using Fluorescence Lifetime Imaging

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Published on: March 27, 2020

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Area of Science:

Biomarkers and Aging Research
Molecular Imaging and Diagnostics
Stress Physiology

Background:

Aging diagnosis typically relies on static biomarkers, which are insufficient for comprehensive evaluation.
Declining stress response capacity (SRC) is a critical hallmark of aging.
Current methods lack dynamic assessment of SRC, limiting preventative intervention strategies.

Purpose of the Study:

To develop a novel method for precisely assessing SRC decay in vivo.
To introduce a dual-channel fluorescent probe for real-time SRC evaluation.
To provide a new strategy for fluorescence-guided aging diagnosis.

Main Methods:

Development of a dual-channel fluorescent probe, ROKS, for simultaneous visualization of thiophenol (stressor) and HOCl (product).
Logic-based fluorescence ratio analysis to quantify SRC from the time dimension.
In vivo imaging of SRC in live cells and Caenorhabditis elegans models.

Main Results:

ROKS successfully visualized thiophenol and HOCl, enabling dynamic SRC assessment.
Observed a decay in SRC with cellular senescence.
Demonstrated higher SRC in young versus aged Caenorhabditis elegans.

Conclusions:

ROKS offers a promising strategy for fluorescence-guided diagnosis of aging.
The probe facilitates precise, time-resolved evaluation of SRC.
This approach aids in assessing the efficacy of anti-aging interventions and drugs.