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Properties of a CCAAT box-binding protein
R A Hooft van Huijsduijnen1, J Bollekens, A Dorn
1Laboratoire de Génétique Moléculaire des Eucaryotes du CNRS, Unité 184 de Biologie Moléculaire et de Génie Génétique de l'INSERM, Faculté de Médecine, Strasbourg, France.
Nucleic Acids Research
|September 25, 1987
Summary
NF-Y, a CCAAT box-binding protein, is found throughout cells and has a large, protease-resistant DNA-binding domain. This ubiquitous transcription factor likely functions as a metallo-protein, offering new insights into its structure and function.
Area of Science:
- Molecular Biology
- Biochemistry
- Genetics
Background:
- NF-Y is a sequence-specific DNA-binding protein.
- It interacts with the Y motif of the major histocompatibility complex class II gene, E alpha.
- NF-Y also binds to CCAAT sequences in other promoters.
Purpose of the Study:
- To investigate the cell-type distribution of NF-Y.
- To characterize the biochemical properties of NF-Y.
- To compare NF-Y with other sequence-specific DNA-binding proteins.
Main Methods:
- Cell-type distribution analysis.
- Biochemical property characterization.
- DNA-binding domain analysis.
Main Results:
- NF-Y is ubiquitously distributed across cell types.
- NF-Y is likely a metallo-protein.
- NF-Y possesses a protease-resistant DNA-binding domain.
- The NF-Y/E alpha-olgo complex is large (>200 kD).
Conclusions:
- NF-Y is a widely distributed transcription factor with unique biochemical properties.
- These findings provide insights into NF-Y's structure and function.
- Further research can compare NF-Y to other DNA-binding proteins.