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Related Experiment Videos

Brain cDNA clone for human cholinesterase.

C McTiernan1, S Adkins, A Chatonnet

  • 1Pharmacology Department, Case Western Reserve University School of Medicine, Cleveland, OH 44106.

Proceedings of the National Academy of Sciences of the United States of America
|October 1, 1987
PubMed
Summary
This summary is machine-generated.

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Researchers identified identical human cholinesterase (EC 3.1.1.8) sequences in both the brain and serum. This suggests a single gene likely codes for this crucial enzyme, impacting neurological and physiological functions.

Area of Science:

  • Molecular Biology
  • Neuroscience
  • Biochemistry

Background:

  • Human serum cholinesterase (EC 3.1.1.8) plays vital roles in neurotransmission and detoxification.
  • Understanding the genetic basis and tissue-specific expression of cholinesterase is crucial for neurological and biochemical research.

Purpose of the Study:

  • To isolate and characterize cDNA clones encoding human serum cholinesterase from the basal ganglia.
  • To compare the amino acid sequence of cholinesterase from the brain with that from serum.
  • To investigate the genetic basis of cholinesterase production.

Main Methods:

  • Screening of a human basal ganglia cDNA library using oligonucleotide probes.
  • Isolation and sequencing of overlapping cDNA clones.
  • Comparison of deduced amino acid sequences with known cholinesterase sequences.

Related Experiment Videos

  • Genomic DNA blot hybridization.

    • Main Results:

    • Five overlapping cDNA clones totaling 2.4 kilobases were isolated.
    • The deduced amino acid sequence precisely matched the known 574-amino acid sequence of human serum cholinesterase.
    • Identical cholinesterase sequences were found in both human brain and serum.
    • Genomic analysis suggests a single gene, or very few genes, encode cholinesterase.

    Conclusions:

    • The amino acid sequences of cholinesterase from the human brain and serum are identical.
    • The isolated cDNA clones represent cholinesterase (EC 3.1.1.8), not acetylcholinesterase (EC 3.1.1.7).
    • A single gene likely codes for human cholinesterase, indicating conserved expression across different tissues.