A ratiometric fluorescence sensor based on carbon quantum dots realized the quantitative and visual detection of Hg²

In this paper, based on the fluorescence of carbon quantum dots (CQDs) quenched by mercury ions (Hg²⁺ ) and the nonresponse of Hg²⁺ to rhodamine B fluorescence, a dual emission ratio fluorescence sensor was constructed to realize the quantitative detection of Hg²⁺ . Under excitation at 365 nm, the fluorescence spectrum showed double emission peaks at 437 nm and 590 nm, corresponding to the fluorescence emissions of CQDs and rhodamine B, respectively. This method quantitatively detected Hg²⁺ based on the linear relationship between the ratio of the intensities of the two emission peaks F₄₃₇ /F₅₉₀ and the concentration of Hg²⁺ . The detection range was 10-70 nM, and the limit of detection (S/N = 3) was 3.3 nM. In addition, this method could also realize the qualitative and semiquantitative detection of Hg²⁺ according to the fluorescence colour change of the probe under ultraviolet light. After various evaluations, the method could be successfully applied to the quantitative and visual detection of Hg²⁺ in tap water, and demonstrated excellent selectivity, anti-interference performance, and repeatability of the method.