Fully Automatic Multi-Class Multi-Residue Analysis of Veterinary Drugs Simultaneously in an Integrated Chip-MS...

Area of Science:

• Analytical Chemistry
• Biotechnology
• Food Science

Background:

• Microfluidic chip analysis offers advantages like high integration, speed, and automation.
• It is widely applied in biological fields, agriculture, and food safety analysis.

Purpose of the Study:

• To develop a fully automated microfluidic chip-mass spectrometry (chip-MS) platform for simultaneous multi-class, multi-residue analysis of veterinary drugs in milk.

Main Methods:

• An integrated microfluidic chip platform was designed, incorporating extraction/filtration, clean-up, and online evaporation modules.
• The chip was coupled to a mass spectrometry detector for simultaneous qualitative and quantitative analysis.
• Analysis was performed without chromatographic separation.

Main Results:

• The system successfully detected 23 veterinary drug residues across five classes in spiked milk samples.
• Limits of detection ranged from 0.23-4.13 ng/mL.
• Recovery rates were between 71.7% and 118.0% under optimized conditions.

Conclusions:

• The developed microfluidic chip system provides a novel approach for veterinary drug residue detection in milk.
• This technology shows significant potential for point-of-care testing applications in the dairy industry.