Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

IR Frequency Region: Fingerprint Region

IR Frequency Region: Fingerprint Region

IR spectra are divided into two main regions: the diagnostic region and the fingerprint region. The diagnostic region of the spectrum lies above 1500 cm−1. The absorptions resulting from single-bond vibrations of the N–H, C–H, and O–H stretch at higher wavenumbers and appear on the left side of the spectrum. The stretching absorptions of the C≡C and C≡N occur between 2100–2300 cm−1. In contrast, those arising from stretching absorptions of the...

Infrared (IR) Spectroscopy: Overview

Infrared (IR) Spectroscopy: Overview

When electromagnetic radiation passes through a material, atoms or molecules transition from a lower to a higher energy state by absorbing radiation corresponding to the energy difference between the two states. The absorption of infrared (IR) radiation causes transitions between vibrational energy levels in a molecule. Therefore, IR spectroscopy is a useful analytical tool for determining the molecular structure of molecules.
Different compounds display unique properties due to their...

Computed Tomography

Computed Tomography

Tomography refers to imaging by sections. Computed tomography (CT) is a non-invasive imaging technique that uses computers to analyze several cross-sectional X-rays to reveal minute details about structures in the body.
The technique was invented in the 1970s and is based on the principle that as X-rays pass through the body, they are absorbed or reflected at different levels. In the technique, a patient lies on a motorized platform while a computerized axial tomography (CAT) scanner rotates...

Imaging Biological Samples with Optical Microscopy

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...

IR Frequency Region: X–H Stretching

IR Frequency Region: X–H Stretching

In IR spectroscopy, signals produced by the X−H bonds (such as C−H, O−H, or N−H) can be observed in the frequency range of 2700–4000 cm–1. The C−H stretching vibration forms sharp bands in the region 2850–3000 cm–1. The presence of the O−H stretching vibration leads to the forming of an absorption band in the frequency range 3650–3200 cm−1. At the same time, N−H stretching can be confirmed by absorption bands in...

IR Spectroscopy: Hooke's Law Approximation of Molecular Vibration

IR Spectroscopy: Hooke's Law Approximation of Molecular Vibration

A covalently bonded heteronuclear diatomic molecule can be modeled as two vibrating masses connected by a spring. The vibrational frequency of the bond can be expressed using an equation derived from Hooke's law, which describes how the force applied to stretch or compress a spring is proportional to the displacement of the spring. In this case, the atoms behave like masses, and the bond acts like a spring.
According to Hooke's law, the vibrational frequency is directly proportional to...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Monitoring of Early Skin Reactions by Optical Coherence Tomography and Dermatoscopy in Patients Receiving Radiation Therapy for Head and Neck Cancer.

Advances in radiation oncology·2025

Same author

Mid-infrared hyperspectral single-pixel microscopy with a quantum cascade laser.

Optics express·2025

Same author

Quantum Fourier Transform Infrared Spectroscopy: Evaluation, Benchmarking, and Prospects.

Applied spectroscopy·2025

Same author

Assessing the Adhesiveness and Long-Term Behaviour of Piezoresistive Strain Sensor Materials for Application in Structural Health Monitored Structures.

Sensors (Basel, Switzerland)·2025

Same author

All-mirror wavefront division interferometer for Fourier transform spectrometry across multiple spectral ranges.

Optics express·2025

Same author

Effects of Particle Shape and Surface Structure on the Adsorption Properties of Polystyrene Microplastics.

Polymers·2024

Search research articles

Related Experiment Video

Updated: Oct 13, 2025

Integrated Photoacoustic Ophthalmoscopy and Spectral-domain Optical Coherence Tomography

Integrated Photoacoustic Ophthalmoscopy and Spectral-domain Optical Coherence Tomography

Published on: January 15, 2013

Time-encoded mid-infrared Fourier-domain optical coherence tomography: erratum.

Ivan Zorin, Paul Gattinger, Andrii Prylepa

|November 15, 2021

Summary

This erratum corrects the labeling of absorption bands in a previous letter, specifically reversing the order for CH vibrations in Fig. 2(a). The scientific conclusions remain unchanged by this correction.

More Related Videos

Doppler Optical Coherence Tomography of Retinal Circulation

Doppler Optical Coherence Tomography of Retinal Circulation

Published on: September 18, 2012

Author Spotlight: Advancements in In Vivo and Ex Vivo Retinal Imaging for Improved Glaucoma Diagnosis and Treatment

Author Spotlight: Advancements in In Vivo and Ex Vivo Retinal Imaging for Improved Glaucoma Diagnosis and Treatment

Published on: June 30, 2023

Related Experiment Videos

Last Updated: Oct 13, 2025

Integrated Photoacoustic Ophthalmoscopy and Spectral-domain Optical Coherence Tomography

Integrated Photoacoustic Ophthalmoscopy and Spectral-domain Optical Coherence Tomography

Published on: January 15, 2013

Doppler Optical Coherence Tomography of Retinal Circulation

Doppler Optical Coherence Tomography of Retinal Circulation

Published on: September 18, 2012

Author Spotlight: Advancements in In Vivo and Ex Vivo Retinal Imaging for Improved Glaucoma Diagnosis and Treatment

Author Spotlight: Advancements in In Vivo and Ex Vivo Retinal Imaging for Improved Glaucoma Diagnosis and Treatment

Published on: June 30, 2023

Area of Science:

Optics
Spectroscopy

Background:

Previous research published in Optics Letters reported findings on absorption bands.
Accurate labeling of spectral features is crucial for scientific reproducibility.

Purpose of the Study:

To correct a labeling error in Fig. 2(a) of a previously published letter.
To ensure accurate representation of CH vibration absorption bands.

Main Methods:

Identification of mislabeled absorption bands in Fig. 2(a).
Correction of the order of CH vibration bands.

Main Results:

The order of absorption bands corresponding to CH vibrations has been reversed in Fig. 2(a).

Conclusions:

The correction of the absorption band labeling does not alter the original scientific conclusions of the letter.