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Visualization of DNA Repair Proteins Interaction by Immunofluorescence
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Nucleolar and spindle associated protein 1 enhances chemoresistance through DNA damage repair pathway in chronic

Yang Han1,2,3,4, Xinting Hu1,2,3,4, Xiaoya Yun1,2,3,4

  • 1Department of Hematology, Shandong Provincial Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong, 250021, China.

Cell Death & Disease
|November 16, 2021
PubMed

Insights

Nucleolar and spindle-associated protein 1 (NUSAP1) is overexpressed in chronic lymphocytic leukemia (CLL), promoting cancer growth and chemoresistance. Silencing NUSAP1 inhibits CLL cell proliferation and enhances treatment sensitivity.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cell Biology

Background:

  • Nucleolar and spindle-associated protein 1 (NUSAP1) is crucial for mitosis and chromosome attachment.
  • NUSAP1 functions as an oncogene in various cancers, but its role in chronic lymphocytic leukemia (CLL) is not well understood.

Purpose of the Study:

  • To investigate the role of NUSAP1 in CLL pathogenesis and its potential as a therapeutic target.
  • To elucidate the mechanism by which NUSAP1 influences CLL cell behavior and chemoresistance.

Main Methods:

  • NUSAP1 expression levels were analyzed in CLL patient samples.
  • NUSAP1 was silenced in CLL cells using lentiviral transfection.
  • Cell proliferation, apoptosis, cell cycle, and DNA damage repair were assessed.
  • In vivo xenograft models were used to evaluate NUSAP1 knockdown effects.
  • Sensitivity to chemotherapy drugs (fludarabine, ibrutinib) was tested.

Main Results:

  • NUSAP1 was significantly overexpressed in CLL and correlated with poor prognosis.
  • NUSAP1 silencing inhibited CLL cell proliferation, induced apoptosis, and caused G0/G1 phase arrest.
  • High NUSAP1 expression enhanced DNA damage repair by stabilizing RAD51.
  • NUSAP1 knockdown suppressed CLL tumor growth in vivo.
  • Reduced NUSAP1 expression increased CLL cell sensitivity to fludarabine and ibrutinib.

Conclusions:

  • NUSAP1 overexpression promotes CLL progression and chemoresistance by enhancing DNA damage repair via RAD51.
  • NUSAP1 is a potential therapeutic target for overcoming chemotherapy resistance in CLL.

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