Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

9.7K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
9.7K
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

17.0K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
17.0K
Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

435
Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
435

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Towards the construction of a virtual yeast.

Nature·2026
Same author

Author Correction: Long-term, in toto live imaging of cardiomyocyte behaviour during mouse ventricle chamber formation at single-cell resolution.

Nature cell biology·2026
Same author

Localizable Fluorescent Metal Ion Indicators With Tunable Colors.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

ER-derived caveolin-coated vesicles transport newly synthesized cholesterol to the plasma membrane.

The Journal of cell biology·2026
Same author

Screening additive for stable solid electrolyte interphase in polymer lithium battery by coulometric titration time analysis.

Journal of colloid and interface science·2026
Same author

A high-affinity split-HaloTag for live-cell protein labeling.

Nature communications·2026

Related Experiment Video

Updated: Oct 13, 2025

Super-Resolution Live Cell Imaging of Subcellular Structures
06:50

Super-Resolution Live Cell Imaging of Subcellular Structures

Published on: January 13, 2021

5.0K

Sparse deconvolution improves the resolution of live-cell super-resolution fluorescence microscopy.

Weisong Zhao1, Shiqun Zhao2, Liuju Li2

  • 1Advanced Microscopy and Instrumentation Research Center, School of Instrumentation Science and Engineering, Harbin Institute of Technology, Harbin, China.

Nature Biotechnology
|November 16, 2021
PubMed
Summary

Researchers developed a new deconvolution algorithm, sparse structured illumination microscopy (Sparse-SIM), to nearly double the resolution of super-resolution microscopes. This advancement enables clearer visualization of live-cell dynamics at high speeds.

More Related Videos

Live Images of GLUT4 Protein Trafficking in Mouse Primary Hypothalamic Neurons Using Deconvolution Microscopy
08:47

Live Images of GLUT4 Protein Trafficking in Mouse Primary Hypothalamic Neurons Using Deconvolution Microscopy

Published on: December 7, 2017

9.9K
Confocal and Super-Resolution Imaging of Polarized Intracellular Trafficking and Secretion of Basement Membrane Proteins During Drosophila Oogenesis
10:41

Confocal and Super-Resolution Imaging of Polarized Intracellular Trafficking and Secretion of Basement Membrane Proteins During Drosophila Oogenesis

Published on: May 19, 2022

2.3K

Related Experiment Videos

Last Updated: Oct 13, 2025

Super-Resolution Live Cell Imaging of Subcellular Structures
06:50

Super-Resolution Live Cell Imaging of Subcellular Structures

Published on: January 13, 2021

5.0K
Live Images of GLUT4 Protein Trafficking in Mouse Primary Hypothalamic Neurons Using Deconvolution Microscopy
08:47

Live Images of GLUT4 Protein Trafficking in Mouse Primary Hypothalamic Neurons Using Deconvolution Microscopy

Published on: December 7, 2017

9.9K
Confocal and Super-Resolution Imaging of Polarized Intracellular Trafficking and Secretion of Basement Membrane Proteins During Drosophila Oogenesis
10:41

Confocal and Super-Resolution Imaging of Polarized Intracellular Trafficking and Secretion of Basement Membrane Proteins During Drosophila Oogenesis

Published on: May 19, 2022

2.3K

Area of Science:

  • Biophysics
  • Cell Biology
  • Microscopy

Background:

  • Live-cell super-resolution (SR) microscopy resolution is limited by photon flux.
  • Biological structures possess inherent sparsity and continuity properties.

Purpose of the Study:

  • To develop a deconvolution algorithm to enhance the spatial resolution of SR microscopes.
  • To leverage a priori knowledge of biological structures for improved imaging.

Main Methods:

  • Developed sparse structured illumination microscopy (Sparse-SIM), a deconvolution algorithm.
  • Applied Sparse-SIM to live-cell imaging, achieving high frame rates.
  • Utilized Sparse-SIM for 3D super-resolution microscopy, including spinning-disc confocal-based SIM.

Main Results:

  • Achieved ~60-nm resolution in live cells at frame rates up to 564 Hz.
  • Resolved intricate cellular structures like fusion pores and nuclear pores.
  • Enabled 3D live-cell SR imaging at ~90-nm resolution, even at low signal-to-noise ratios.

Conclusions:

  • Sparse deconvolution significantly enhances the effective resolution of SR microscopes.
  • Sparse-SIM offers a powerful tool for high-spatiotemporal resolution live-cell imaging.
  • The method improves visualization of dynamic cellular processes and structures.