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Influence of various factors on binding of 67Ga to polymorphonuclear leukocytes
R E Weiner1, M S Cohen, P B Hoffer
1Department of Diagnostic Radiology, University of Kansas College of Health Sciences and Hospital, Kansas City 66103.
International Journal of Radiation Applications and Instrumentation. Part B, Nuclear Medicine and Biology
|January 1, 1987
Abstract:
We have studied in vitro, factors that influence the uptake of 67Ga-citrate by human polymorphonuclear leukocytes. Citrate at 20 mM concentration decreased the uptake to 1% of control values. Uptake increased as a function of increased microCi of 67Ga/10(7) cells added and incubation time from 0 to 120 min. Uptake decreased somewhat as the incubation pH was lowered from 7.4 to 6.0. Our results suggest that, in vivo, these cells would accumulate 67Ga as the inflammatory lesion progresses while the acidic milieu would modestly reduce uptake.