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Related Experiment Video

Updated: Oct 13, 2025

Stimulation of Vascular Endothelial Cells Using Neutrophil Extracellular Traps in the Presence of Low-Density Lipoprotein
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Octyl gallate decrease lymphocyte activation and regulates neutrophil extracellular traps release.

Gabriela Viegas Haute1, Carolina Luft1,2, Leonardo Pedrazza3

  • 1Laboratório de Pesquisa em Biofísica Celular e Inflamação, Departamento de Biologia Celular e Molecular, Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Avenida Ipiranga 6681, prédio 12, bloco C, sala 221, Porto Alegre, CEP 90619-900, Brazil.

Molecular Biology Reports
|November 16, 2021
PubMed
Summary

Octyl gallate (OG) inhibits neutrophil extracellular trap (NET) formation and reactive oxygen species (ROS) release, while also modulating lymphocyte proliferation. However, OG did not improve survival in a sepsis model.

Keywords:
ApoptosisImmunomodulationNETosisNeutrophilsOctyl gallateSepsis

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Area of Science:

  • Immunology
  • Pharmacology
  • Cell Biology

Background:

  • Inflammation involves neutrophils, which can undergo apoptosis or NETosis to eliminate pathogens.
  • Neutrophil extracellular traps (NETs) are released during inflammation, a process that must be regulated to prevent self-damage.
  • Octyl gallate (OG), a gallic acid derivative, exhibits antioxidant and anti-inflammatory properties.

Purpose of the Study:

  • To investigate the effects of Octyl gallate (OG) on lymphocyte proliferation and neutrophil activation.
  • To evaluate the efficacy of OG in an experimental model of sepsis.

Main Methods:

  • Human lymphocytes and neutrophils were isolated from healthy donors.
  • Assays included cell viability, apoptosis, NETs release, and antioxidant capacity of OG.
  • Survival was assessed in a C57BL/6 mouse model of experimental sepsis.

Main Results:

  • OG inhibited reactive oxygen species (ROS) release and NETs formation in human neutrophils.
  • OG modulated the effects of lipopolysaccharide (LPS) on neutrophil apoptosis and inhibited peripheral blood mononuclear cell (PBMC) proliferation.
  • No significant increase in survival was observed in septic mice treated with OG.

Conclusions:

  • Octyl gallate demonstrates pharmacological potential by modulating neutrophil and lymphocyte activity.
  • OG's anti-inflammatory effects suggest its possible use as an adjuvant therapy for inflammatory diseases.