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A spectroscopic probe for hypochlorous acid detection.

Ning An1, Dan Wang1, Hui Zhao1

  • 1State Key Laboratory Breeding Base of Green Chemistry Synthesis Technology, College of Chemical Engineering, Zhejiang University of Technology, Hangzhou 310032, China.

Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
|November 17, 2021
PubMed
Summary
This summary is machine-generated.

A new probe, CMBT, detects hypochlorous acid (HClO) with turn-on blue fluorescence and a visible color change. This method is effective for water samples and offers a simple paper test strip for HClO detection.

Keywords:
Colorimetric fluorescent probeHypochlorousLogic circuitNaked-eye recognitionTest paper

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Area of Science:

  • Analytical Chemistry
  • Spectroscopy
  • Chemical Sensing

Background:

  • Hypochlorous acid (HClO) is a reactive oxygen species crucial in biological processes and environmental monitoring.
  • Developing selective and sensitive probes for HClO detection is essential for various applications.

Purpose of the Study:

  • To synthesize and characterize a novel spectroscopic probe, CMBT, for the detection of HClO.
  • To investigate the sensing mechanism and evaluate the performance of CMBT for HClO detection.

Main Methods:

  • Synthesis and characterization of the CMBT probe.
  • Spectroscopic analysis (fluorescence and UV-vis) for HClO detection.
  • Evaluation of detection limits and interference studies.
  • Application in real water samples and development of paper test strips.

Main Results:

  • CMBT exhibited specific recognition for HClO, showing turn-on blue fluorescence and a naked-eye color change from pink to colorless.
  • The sensing mechanism involves the oxidation of CMBT by HClO, leading to fluorescence recovery.
  • Low detection limits were achieved (1.61 μM for fluorescence, 6.58 μM for UV-vis).
  • Successful detection of HClO in tap and river water samples using CMBT and paper test strips.

Conclusions:

  • CMBT is a highly effective spectroscopic probe for selective and sensitive detection of HClO.
  • The developed probe and paper test strips offer a convenient and practical method for HClO monitoring in environmental samples.
  • The study demonstrates the potential for constructing molecular logic circuits using this sensing system.