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Updated: Oct 13, 2025

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AATF is Overexpressed in Human Head and Neck Squamous Cell Carcinoma and Regulates STAT3/Survivin Signaling

Lin Fu1,2, Quanxiu Jin3, Qianze Dong1,2

  • 1Department of Pathology, College of Basic Medical Science, China Medical University, Shenyang, People's Republic of China.

Oncotargets and Therapy
|November 17, 2021
PubMed
Abstract

Insights

Apoptosis antagonizing transcription factor (AATF) is elevated in head and neck squamous cell carcinoma (HNSCC), promoting cisplatin resistance and reducing apoptosis. Targeting AATF may offer a new therapeutic strategy for HNSCC patients.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cancer Research

Background:

  • Dysregulation of apoptosis antagonizing transcription factor (AATF) is linked to various cancers.
  • The role of AATF in human head and neck squamous cell carcinoma (HNSCC) requires further investigation.

Purpose of the Study:

  • To investigate the expression patterns of AATF in HNSCC.
  • To elucidate the biological functions and underlying mechanisms of AATF in HNSCC progression and treatment response.

Main Methods:

  • Immunohistochemistry on 119 HNSCC samples.
  • Cell proliferation, apoptosis assays (CCK-8, colony formation, Annexin V/PI), Western blotting, and RNA-sequencing.
  • Analysis of TCGA and Oncomine databases for AATF expression and correlation with patient survival.

Main Results:

  • AATF expression is upregulated in HNSCC tissues and correlates with advanced stage and poorer survival.
  • AATF overexpression enhances cell growth, colony formation, and cisplatin resistance, while reducing apoptosis.
  • AATF regulates STAT3/survivin signaling pathway, influencing apoptosis and chemoresistance.

Conclusions:

  • AATF is overexpressed in HNSCC and promotes cisplatin resistance and reduced apoptosis.
  • The STAT3/survivin signaling pathway is implicated in AATF's function.
  • AATF represents a potential therapeutic target for HNSCC treatment.
Keywords:
AATFSTAT3head and neck squamous cell carcinomasurvivin

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