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Dynamic Urinary Proteome Changes in Ovalbumin-Induced Asthma Mouse Model Using Data-Independent Acquisition
Weiwei Qin1,2, Ting Wang2, Guangwei Liu3
1Department of Anesthesiology, Qingdao Municipal Hospital, Qingdao University, Qingdao, 266071, People's Republic of China.
Journal of Asthma and Allergy
|November 17, 2021
Summary
This study identified dynamic changes in urinary proteins during asthma development in a mouse model. These findings suggest potential urinary protein biomarkers for early asthma screening and diagnosis of asthma attacks.
Area of Science:
- Biochemistry
- Immunology
- Proteomics
Background:
- Asthma is a chronic respiratory disease with complex pathophysiology.
- Identifying reliable biomarkers for asthma diagnosis and progression is crucial.
Purpose of the Study:
- To investigate dynamic changes in the urinary proteome during asthma development.
- To identify potential urinary protein biomarkers for asthma diagnosis and monitoring.
Main Methods:
- An ovalbumin (OVA)-induced mouse model was utilized to simulate asthma.
- Urinary proteome analysis was performed using data-independent acquisition and high-resolution tandem mass spectrometry.
Main Results:
- 53 differentially expressed proteins were identified in the urine of asthma model mice.
- Enriched pathways included acute-phase response, innate immune response, and complement activation.
- Specific proteins were associated with early asthma development and diagnosis of asthma attacks.
Conclusions:
- The urinary proteome dynamically reflects pathophysiological changes during asthma progression.
- Urinary proteins show potential as biomarkers for early asthma detection and diagnosis of asthma exacerbations.
