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Updated: Oct 13, 2025
Leprdb Mouse Model of Type 2 Diabetes: Pancreatic Islet Isolation and Live-cell 2-Photon Imaging Of Intact Islets
Published on: May 11, 2015
LncRNA PTGS2 regulates islet β-cell function through the miR-146a-5p/RBP4 axis and its diagnostic value in type 2
Qian Chen1,2, Yun He1,2, Xufeng Wang1,2
1Department of Gastrointestinal Surgery, Hubei Provincial Hospital of Traditional Chinese Medicine Wuhan 430061, Hubei Province, China.
Objective:
To investigate the effect of long non-coding RNA (LncRNA) PTGS2 on islet β-cell function via the miR-146a-5p/Retinol binding protein 4 (RBP4) axis and its diagnostic value in type 2 diabetes mellitus (T2DM).
Methods:
The Gene Expression Omnibus (GEO) was analyzed and LncRNA PTGS2 was identified as a potential regulator of T2DM. Mouse pancreatic β cell INS-1 cells were cultured with high glucose, and the relative expression of LncRNA PTGS2 in the serum of T2DM patients and INS-1 cells was detected by Fluorescence Quantitative PCR (qRT-PCR) and its diagnostic value for T2DM was analyzed. The PTGS2/miR-146a-5p/RBP4 axis in INS-1 cells was intervened to observe the changes in cell function. The proliferation of INS-1 cells was detected by CCK8, and the level of insulin secretion was detected by enzyme linked immunosorbent assay (ELISA). The regulatory relationship among LncRNA PTGS2, miR-146a-5p and RBP4 was determined by dual-luciferase reporter assay.
Results:
The expression of LncRNA PTGS2 in the serum of T2DM patients increased, and the expression of LncRNA PTGS2 was positively correlated with the fasting blood glucose level of patients (R=0.306, P<0.05). Knockdown of LncRNA PTGS2 could promote the proliferation and insulin secretion of INS-1 cells, while overexpression of LncRNA PTGS2 showed the opposite results (all P<0.05). Knockdown of LncRNA PTGS2 could up-regulate the expression of miR-146a-5p. Overexpression of LncRNA PTGS2 inhibited the proliferation and insulin secretion of INS-1 cells, while miR-146a-5p could partially reverse this effect. RBP4 has been identified as a downstream target gene of miR-146a-5p. Overexpression of miR-146a-5p could inhibit the expression of RBP4, which was positively correlated withLncRNA PTGS2 regulation. The effect of RBP4 on INS-1 cells was the same as that of LncRNA PTGS2.
Conclusion:
LncRNA PTGS2 can damage islet β-cell function by regulation of miR-146a-5p and up-regulation of RBP4. LncRNA PTGS2 has potential value in the diagnosis of T2DM.
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