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Related Experiment Videos

Binary liquid phase separation and critical phenomena in a protein/water solution.

J A Thomson1, P Schurtenberger, G M Thurston

  • 1Department of Physics, Massachusetts Institute of Technology, Cambridge 02139.

Proceedings of the National Academy of Sciences of the United States of America
|October 1, 1987
PubMed
Summary

Aqueous solutions of bovine gamma II-crystallin protein exhibit liquid-liquid phase separation below 278.5 K. This protein system serves as a model for studying critical phenomena and phase transitions.

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Area of Science:

  • Biophysics
  • Physical Chemistry
  • Materials Science

Background:

  • Understanding protein solution behavior is crucial for biological and industrial applications.
  • Phase transitions in protein solutions can lead to aggregation and loss of function.

Purpose of the Study:

  • To investigate the phase diagram of aqueous solutions of bovine gamma II-crystallin.
  • To characterize the liquid-liquid phase separation and crystallization phenomena.

Main Methods:

  • Measurements of coexisting concentrations and cloud temperatures.
  • Analysis of scattered light intensity to determine the spinodal line.
  • Observation of protein crystallization.

Main Results:

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  • A reversible liquid-liquid phase coexistence was observed below 278.5 K.
  • The coexistence curve and spinodal line were characterized, with ratios consistent with mean-field theory.
  • Crystallization of gamma II-crystallin was observed, with preliminary factors influencing its onset identified.

    • Conclusions:

    • Aqueous solutions of gamma II-crystallin exhibit critical phenomena and phase transitions.
    • These protein/water systems are suitable models for studying phase transitions.
    • Further research into crystallization factors is warranted.