Pb contaminated soil influence on aboveground biomass and bioactive compounds in leaves of mulberry

This study investigated the effects of soil lead (Pb), biochar, and partial root zone drought (PRD) on mulberry (Morus alba L.) branches and leaves biomass, Pb accumulation, and bioactive compounds including 1-deoxynojirimycin (DNJ) and flavonoids. Three-factor pot experiments were conducted with biochar, PRD, and soil Pb at four concentration levels (0, 50, 200, and 800 mg kg^-1). Results revealed that mulberry aboveground biomass did not decrease significantly across the soil Pb levels. Pb concentration of mulberry leaves do not increase significantly when soil Pb was 200 mg kg^-1; however, it significantly accumulated under 800 mg kg^-1. There was a dose-effect between the Pb concentration in branches and the soil Pb levels. Mulberry leaf flavonoids were affected by the interaction of soil Pb and biochar. The interaction between two of the three factors significantly affected leaves DNJ concentration. The combination of biochar and PRD maintained the biomass of mulberry and did not significantly increase Pb in leaves under 200 mg kg^-1 soil Pb concentration. In summary, mulberry has a higher resistance to soil Pb stress, and it can be planted in moderate Pb-contaminated soils for no loss of biomass and can safely harvest the branches and leaves.Novelty statementAn economic benefit is a key to the practical application and sustainability of phytoremediation. Based on this, we studied the effects of soil Pb on biomass, Pb accumulation, and bioactive substance concentration of harvesting organs in mulberry.Phytoremediation is not isolated, and techniques, such as soil amendments and water management also play a role. In this study, we found that biochar and partial root-zone irrigation had a synergistic effect on the response of mulberry to soil Pb, which could be co-applied in the phytoremediation of lead-contaminated soil.The concentration of heavy metals is the key to ensuring product safety in heavy metal contaminated soil. We found that Pb concentration in leaf and stem of mulberry did not significantly increase under 200 mg kg^-1 soil Pb, while increased at 800 mg kg^-1 soil Pb. Therefore, planting mulberry on 200 mg kg^-1 Pb contaminated soil can safely harvest branches and leaves.