Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Raft Affinity and Membrane Modulation of Docosahexaenoic Acid-Containing Phospholipids Revealed by Comparative Analysis Using Fluorescently Labeled Lipids.

Langmuir : the ACS journal of surfaces and colloids·2026
Same author

Structural Analysis of Gel-Phase-Like Subdomains Formed in Lipid-Raft-Mimetic Bilayers.

Langmuir : the ACS journal of surfaces and colloids·2026
Same author

Quantitative Mapping of the Lipid Nanoenvironment around Transmembrane Proteins in Living Cells.

ACS nano·2026
Same author

Unraveling the Disruptive Mechanism of Local Anesthetics on Raft-Like Ordered Membranes: Simulation Studies.

Journal of chemical information and modeling·2025
Same author

Quantifying Cell Membrane Permeability of Small to Mid-Size Drugs Using Quick Osmotic Cytoplasm Extraction.

Analytical chemistry·2025
Same author

Simple and rapid TLC blotting-based methodology for screening membrane protein-specific lipids.

Analytical sciences : the international journal of the Japan Society for Analytical Chemistry·2025

Related Experiment Video

Updated: Oct 13, 2025

Sample Preparation for Rapid Lipid Analysis in Drosophila Brain Using Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging
09:00

Sample Preparation for Rapid Lipid Analysis in Drosophila Brain Using Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging

Published on: July 14, 2022

3.4K

Recent advances in microscale separation techniques for lipidome analysis.

Takayuki Kawai1, Nobuaki Matsumori1, Koji Otsuka2

  • 1Department of Chemistry, Faculty of Science, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan. takayuki.kawai@chem.kyushu-univ.jp.

The Analyst
|November 17, 2021
PubMed
Summary

Microscale separation techniques, including capillary liquid chromatography (LC) and capillary electrophoresis (CE), enhance lipidome analysis by improving resolution and sensitivity when coupled with mass spectrometry (MS). These methods are crucial for detailed lipid identification and are advancing lipidomics research.

More Related Videos

Shotgun Lipidomics of Rodent Tissues
11:46

Shotgun Lipidomics of Rodent Tissues

Published on: November 18, 2022

2.2K
A Simple Fractionated Extraction Method for the Comprehensive Analysis of Metabolites, Lipids, and Proteins from a Single Sample
11:17

A Simple Fractionated Extraction Method for the Comprehensive Analysis of Metabolites, Lipids, and Proteins from a Single Sample

Published on: June 1, 2017

35.7K

Related Experiment Videos

Last Updated: Oct 13, 2025

Sample Preparation for Rapid Lipid Analysis in Drosophila Brain Using Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging
09:00

Sample Preparation for Rapid Lipid Analysis in Drosophila Brain Using Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging

Published on: July 14, 2022

3.4K
Shotgun Lipidomics of Rodent Tissues
11:46

Shotgun Lipidomics of Rodent Tissues

Published on: November 18, 2022

2.2K
A Simple Fractionated Extraction Method for the Comprehensive Analysis of Metabolites, Lipids, and Proteins from a Single Sample
11:17

A Simple Fractionated Extraction Method for the Comprehensive Analysis of Metabolites, Lipids, and Proteins from a Single Sample

Published on: June 1, 2017

35.7K

Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Separation Science

Background:

  • Lipids are vital biomolecules involved in cellular functions.
  • Lipidome analysis is challenging due to lipid complexity and isomerism.
  • Mass spectrometry (MS) is key for lipid identification but requires prior separation.

Purpose of the Study:

  • To review microscale liquid-phase separation techniques for lipidome analysis.
  • To focus on capillary LC and CE coupled with MS over the past decade.
  • To highlight the advancements and potential of these techniques.

Main Methods:

  • Review of recent research (last 10 years) on microscale separation.
  • Focus on capillary liquid chromatography (LC) and capillary electrophoresis (CE).
  • Integration with mass spectrometry (MS) for enhanced detection.

Main Results:

  • Microscale separation (capillary/microchip LC and CE) offers high-resolution separation.
  • nL-scale flow is compatible with sensitive nanoelectrospray ionization-MS.
  • Over 100 lipidome studies have utilized these microscale techniques.
  • Techniques are evolving for higher resolution and wider lipidome coverage.

Conclusions:

  • Microscale separation techniques are essential for overcoming lipidome analysis challenges.
  • These methods provide high sensitivity and resolution for lipid identification.
  • Microscale separation is a promising foundational technology for future lipidomics.