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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Considerations for user consultation in a flow cytometry shared resource laboratory.

Amy Graham¹, Jena Korecky¹, Eric Schultz¹

¹Flow Cytometry Core Lerner Research Institute, Cleveland Clinic, Cleveland, Ohio, USA.

Cytometry. Part a : the Journal of the International Society for Analytical Cytology

|November 17, 2021

Summary

Implementing a structured user consultation platform in shared resource laboratories (SRLs) is key for high-quality flow cytometry data. This approach guides researchers and enhances experimental rigor and reproducibility.

Keywords:

communication documented report flow cytometry panel design recommendations sample preparation shared resource lab user consultation

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Area of Science:

Biomedical Research
Laboratory Science
Data Acquisition

Background:

High-quality flow cytometric data relies heavily on effective user consultation within shared resource laboratories (SRLs).
SRL staff face challenges in guiding diverse users to meet specific experimental needs.
A standardized consultation process is needed to bridge the gap between experimental conception and data acquisition.

Purpose of the Study:

To outline a structured user consultation platform for SRLs.
To guide SRL staff in understanding researcher goals and providing expert advice.
To enhance communication and improve experimental rigor in flow cytometry.

Main Methods:

Describing considerations for SRLs to understand researcher objectives.
Detailing how SRL staff can offer structured expert advice.
Highlighting the educational component of consultations on best practices.

Main Results:

A structured consultation platform addresses critical steps from experiment conception to sample acquisition.
Consultations inform users about current best practices in cytometry.
Consultation reports improve communication among SRLs, principal investigators, and research teams.

Conclusions:

Implementing a standardized user consultation platform is essential for SRLs.
This structured approach enhances the quality, rigor, and reproducibility of flow cytometric data.
Best practices in user consultation are being developed by the ISAC SRL committee.