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Multiwindow SRS Imaging Using a Rapid Widely Tunable Fiber Laser.

Hongli Ni1, Peng Lin1, Yifan Zhu2

  • 1Department of Electrical and Computer Engineering, Boston University, 8 St. Mary's Street, Boston, Massachusetts 02215, United States.

Analytical Chemistry
|November 17, 2021
PubMed
Summary

A new compact fiber laser system enables rapid, multi-window stimulated Raman scattering (SRS) imaging. This advancement overcomes limitations of bulky lasers, improving signal-to-noise for biological and chemical analysis.

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Area of Science:

  • Biomedical Optics
  • Spectroscopy
  • Microscopy

Background:

  • Spectroscopic stimulated Raman scattering (SRS) imaging is valuable but limited by bulky, sensitive solid-state optical parametric oscillators (OPOs).
  • Current SRS systems face challenges in rapid, multi-spectral imaging due to slow OPO wavelength tuning, hindering broader adoption.

Purpose of the Study:

  • To develop a compact, robust multi-window SRS imaging system with rapid wavelength tuning.
  • To enhance signal-to-noise ratio (SNR) for stimulated Raman loss (SRL) imaging using fiber laser technology.

Main Methods:

  • A novel SRS microscope utilizing a compact fiber laser with wide and rapid tuning capabilities was designed.
  • Autobalanced detection was implemented to mitigate relative intensity noise inherent in fiber lasers.
  • The system was tested for multi-window SRS imaging across C-H, C-D, and fingerprint Raman regions.

Main Results:

  • The fiber laser-based SRS system demonstrated rapid and wide wavelength tuning.
  • Autobalanced detection improved the SNR of SRL imaging by 23 times.
  • High-quality SRS metabolic imaging was achieved for fungi, cancer cells, and Caenorhabditis elegans.

Conclusions:

  • The developed compact multi-window SRS system offers a robust and efficient alternative to existing technologies.
  • This advancement facilitates broader applications of SRS imaging in biological and chemical analysis.
  • The system shows significant potential for diverse research fields requiring detailed chemical information.