Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Capillary Electrophoresis: Instrumentation01:20

Capillary Electrophoresis: Instrumentation

461
Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...
461
Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

607
Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
607

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Experimental Observations of DNA Vertex Pinning: Effect of Adsorbed Polymer Type and Electric Field Reversal.

Langmuir : the ACS journal of surfaces and colloids·2026
Same author

Reaction Kinetics of CRISPR <i>trans</i>-Cleavage Controlled Using Isotachophoresis.

Analytical chemistry·2025
Same author

Microfluidic networks using isotachophoresis.

Proceedings of the National Academy of Sciences of the United States of America·2025
Same author

Engineering guidelines for CRISPR diagnostics.

Chemical communications (Cambridge, England)·2025
Same author

TSPEAR S475TfsX79 mutation does not affect auditory function, tooth morphology or hair development in mice.

Gene·2025
Same author

Degradation of Reporter Molecules Imposes a Fundamental Limit of Detection on CRISPR Diagnostics.

Analytical chemistry·2025
Same journal

Strain-Level Food Surveillance of <i>Escherichia coli</i> Using a Specific-Nonspecific Hybrid Sensor Array Strategy.

Analytical chemistry·2026
Same journal

A Field-Portable Fe(IV)-Mediated Competitive Quenching Chemiluminescence Platform with a Synchronous Y-Shaped Flow-through Cell for Broad-Spectrum Quantification of Volatile Phenols.

Analytical chemistry·2026
Same journal

Single-Molecule Characterization of CRISPR-Cas12a for Amplification-Free Genetic Testing.

Analytical chemistry·2026
Same journal

Integrated Acoustofluidic Manipulation and Oscillation-Stabilized Magnetic Relaxation Biosensing for <i>Salmonella</i> Detection.

Analytical chemistry·2026
Same journal

A Self-Powered Sensing Platform Based on the Janus Heterostructure for Machine Learning-Assisted Dual-Mode Detection of 17β-Estradiol.

Analytical chemistry·2026
Same journal

Large Language Model-Generated Dietary Metabolite Biomarker Database Drives Deep Annotation of the Human Diet Metabolome.

Analytical chemistry·2026
See all related articles

Related Experiment Video

Updated: Oct 13, 2025

On-chip Isotachophoresis for Separation of Ions and Purification of Nucleic Acids
10:32

On-chip Isotachophoresis for Separation of Ions and Purification of Nucleic Acids

Published on: March 2, 2012

24.7K

Web-Based Open-Source Tool for Isotachophoresis.

Alexandre S Avaro1, Yixiao Sun1, Kaiying Jiang1

  • 1Department of Mechanical Engineering, Stanford University, Stanford, California 94305, United States.

Analytical Chemistry
|November 17, 2021
PubMed
Summary
This summary is machine-generated.

A new web-based simulator, CAFES, enables rapid, accurate simulations of complex electrophoresis and isotachophoresis phenomena. This open-source tool requires no installation and validates well against experimental data.

More Related Videos

Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization
12:11

Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization

Published on: February 27, 2020

7.0K
Online Size-exclusion and Ion-exchange Chromatography on a SAXS Beamline
11:09

Online Size-exclusion and Ion-exchange Chromatography on a SAXS Beamline

Published on: January 5, 2017

17.5K

Related Experiment Videos

Last Updated: Oct 13, 2025

On-chip Isotachophoresis for Separation of Ions and Purification of Nucleic Acids
10:32

On-chip Isotachophoresis for Separation of Ions and Purification of Nucleic Acids

Published on: March 2, 2012

24.7K
Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization
12:11

Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization

Published on: February 27, 2020

7.0K
Online Size-exclusion and Ion-exchange Chromatography on a SAXS Beamline
11:09

Online Size-exclusion and Ion-exchange Chromatography on a SAXS Beamline

Published on: January 5, 2017

17.5K

Area of Science:

  • Computational chemistry
  • Analytical chemistry
  • Chemical engineering

Background:

  • Electrophoresis and isotachophoresis are crucial separation techniques.
  • Simulating complex electrolyte behavior, including reactions and transport, is computationally challenging.
  • Existing simulation tools often require installation and significant computation time.

Purpose of the Study:

  • To develop a user-friendly, web-based simulator for complex electrophoresis phenomena.
  • To enable rapid and accurate simulations of coupled unsteady electromigration, diffusion, and electrolyte reactions.
  • To provide a validated tool for predicting ion concentration dynamics and pH profiles.

Main Methods:

  • Development of a client-side JavaScript-based simulator (CAFES).
  • Implementation of a stationary grid and adaptive time step for efficient computation.
  • Inclusion of a database of species and their physicochemical properties.
  • Validation against experimental data for peak- and plateau-mode isotachophoresis.

Main Results:

  • CAFES accurately predicts interface velocity, plateau length, relative intensity, and pH variations.
  • The simulation tool demonstrates significantly reduced computation time compared to existing methods.
  • The simulator offers broad cross-browser compatibility and requires no prior installation or compilation.
  • Validation against experimental isotachophoresis data confirms the accuracy of CAFES predictions.

Conclusions:

  • CAFES provides a powerful, accessible, and efficient tool for simulating complex electrophoresis.
  • The open-source simulator facilitates research and development in microfluidics and analytical chemistry.
  • Web-based simulation reduces barriers to entry for researchers and educators in electrophoresis.