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Updated: Oct 13, 2025
Photodiode-Based Optical Imaging for Recording Network Dynamics with Single-Neuron Resolution in Non-Transgenic Invertebrates
Published on: July 9, 2020
Diesel2p mesoscope with dual independent scan engines for flexible capture of dynamics in distributed neural
Che-Hang Yu1, Jeffrey N Stirman2, Yiyi Yu1
1Department of Electrical and Computer Engineering, University of California, Santa Barbara, CA, USA.
Abstract:
Imaging the activity of neurons that are widely distributed across brain regions deep in scattering tissue at high speed remains challenging. Here, we introduce an open-source system with Dual Independent Enhanced Scan Engines for Large field-of-view Two-Photon imaging (Diesel2p). Combining optical design, adaptive optics, and temporal multiplexing, the system offers subcellular resolution over a large field-of-view of ~25 mm2, encompassing distances up to 7 mm, with independent scan engines. We demonstrate the flexibility and various use cases of this system for calcium imaging of neurons in the living brain.
