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Gene-targeted Random Mutagenesis to Select Heterochromatin-destabilizing Proteasome Mutants in Fission Yeast

Gene-targeted Random Mutagenesis to Select Heterochromatin-destabilizing Proteasome Mutants in Fission Yeast

Published on: May 15, 2018

Segregating Complete Tf2 Elements Are Largely Neutral in Fission Yeast.

Yan Wang¹, Qin Wang¹, Zhiwei Wu¹

¹College of Life Sciences, Nanjing Normal University, Nanjing, China.

Genome Biology and Evolution

|November 18, 2021

Summary

Transposable elements (TEs) are abundant in eukaryote genomes. Our study shows that segregating Tf2 elements in fission yeast do not impact host fitness or gene expression, suggesting they are neutral.

Keywords:

fitness selfish DNA transposable elements yeast

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A Deep-sequencing-assisted, Spontaneous Suppressor Screen in the Fission Yeast Schizosaccharomyces pombe

A Deep-sequencing-assisted, Spontaneous Suppressor Screen in the Fission Yeast Schizosaccharomyces pombe

Published on: March 7, 2019

Area of Science:

Genetics
Genomics
Molecular Biology

Background:

Transposable elements (TEs) constitute a significant portion of eukaryotic genomes.
The impact of TEs on host fitness remains incompletely understood.
Long terminal repeat retrotransposons are a major class of TEs.

Purpose of the Study:

To empirically investigate the influence of TEs on host fitness in the fission yeast Schizosaccharomyces pombe.
To assess the impact of Tf2 elements on gene expression and host response to stress.

Main Methods:

Identification and characterization of Tf2 elements in S. pombe.
Generation of Tf2 knockout strains.
Assessment of host fitness, gene expression, and growth rates under stress conditions.

Main Results:

Two TE families, Tf1 and Tf2, are highly polymorphic in S. pombe.
Thirteen complete Tf2 elements were identified as recently integrated and segregating in the population.
Knockout of Tf2 elements did not affect host fitness, gene expression, or growth rates under stress.

Conclusions:

Segregating complete Tf2 element insertions are largely neutral to host fitness in fission yeast.
TEs in fission yeast exhibit characteristics supporting their selfish nature.
This study provides empirical, genome-wide evidence for TE neutrality in S. pombe.