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Updated: Oct 13, 2025

Measuring Interactions of Globular and Filamentous Proteins by Nuclear Magnetic Resonance Spectroscopy NMR and Microscale Thermophoresis MST
Published on: November 2, 2018
Analysis of protein-ligand interactions from titrations and nuclear magnetic resonance relaxation dispersions
Emil Björklund1, Anna du Rietz2, Patrik Lundström1
1Division of Chemistry, Department of Physics, Chemistry and Biology, Linköping University, Linköping, Sweden.
Abstract:
We present PLIS, a publicly available, open-source software for the determination of protein-ligand dissociation constants that can be used to characterize biological processes or to shed light on biophysical aspects of interactions. PLIS can analyze data from titration experiments monitored by for instance fluorescence spectroscopy or from nuclear magnetic resonance relaxation dispersion experiments. In addition to analysis of experimental data, PLIS includes functionality for generation of synthetic data, useful for understanding how different parameters effect the data in order to better analyze experiments.
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