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Updated: Oct 13, 2025

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Layer-by-layer coating and chemical cross-linking of multilayer polysaccharides on silica for mixed-mode HPLC
Zhanying Chu1, Manman Zhu1, Weibing Zhang2
1Technology Innovation Center of Mass Spectrometry for State Market Regulation, Center for Advanced Measurement Science, National Institute of Metrology, Beijing, 100029, P. R. China. zhairui@nim.ac.cn.
Abstract:
A facile, controllable and environmentally friendly method for fabricating a novel polysaccharide-silica composite stationary phase (SiO2@(HA-CS)12) was developed in this report. Two natural polysaccharides (hyaluronan acid and chitosan) were controllably coated on the silica surface using a layer-by-layer assembly technique, and then the polysaccharide shell was chemically cross-linked to improve the stability. The column efficiency of the SiO2@(HA-CS)12 column reached 74 000 plates per m in HILIC mode and 20 100 plates per m in IEC mode, which indicates great potential for separating polar and charged samples.
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