High performance liquid chromatographic method with post-column detection for quantification of reducing sugars in
Esin Akyüz1, Kevser Sözgen Başkan1, Esma Tütem1
1Department of Chemistry, Faculty of Engineering, İstanbul University-Cerrahpaşa, 34320 Avcılar-İstanbul, Turkey.
Journal of Chromatography. A
|November 18, 2021
Summary
A new liquid chromatography method offers faster, more sensitive sugar analysis in foods. This improved technique uses a novel post-column reaction for accurate detection of various sugars.
Area of Science:
- Analytical Chemistry
- Food Science
Background:
- Existing sugar analysis methods often lack speed, selectivity, or sensitivity.
- There is a need for improved analytical techniques for accurate sugar quantification in food products.
Purpose of the Study:
- To develop a novel liquid chromatographic analysis method with post-column detection for sugars.
- To enhance operational time, selectivity, and sensitivity compared to existing methods.
Main Methods:
- Separation of reducing sugars on an HPLC column at 30°C.
- Post-column reaction with Cu(II)-neocuproine (Nc) reagent at 80°C.
- Measurement of the Cu(I)-Nc product at 450 nm.
Main Results:
- The method accurately quantified glucose, fructose, maltose, and lactose in various food samples (honey, apple juice, milk).
- Non-reducing sucrose was determined indirectly with a relative error from -2.41 to 2.09%.
- High accuracy was confirmed with recovery rates between 96-107% and correlation coefficients > 0.996.
Conclusions:
- The developed method provides a sensitive, selective, and efficient approach for sugar analysis in food.
- It offers advantages over existing methods, including lower post-column reaction temperature and use of low-cost reagents.
- This assay is suitable for routine analysis of sugars in diverse food matrices.
More Related Videos
Related Concept Videos
01:15High-Performance Liquid Chromatography: Types of Detectors
931
The role of the detectors in High-Performance Liquid Chromatography (HPLC) is to analyze the solutes as they exit from the chromatographic column. The detector recognizes the solute's property and generates corresponding electrical signals, which are converted into a readable graph of the detector's response versus elution time called a chromatogram at the computer. There are several types of HPLC detectors, each with its own advantages and limitations, depending on the analyte...
931
01:11High-Performance Liquid Chromatography: Introduction
2.5K
High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
In HPLC, two phases play a critical role in the separation process:
In HPLC, two phases play a critical role in the separation process:
2.5K
00:57High-Performance Liquid Chromatography: Instrumentation
2.2K
High-performance liquid chromatography, or HPLC, is an analytical technique that separates liquid samples under high pressures. An HPLC instrument consists of glass bottles for storing solvents called mobile phase reservoirs. HPLC-grade solvents are used to maintain high purity, and the dissolved gases are removed using a degasser, such as a vacuum pumping system or sparging with helium. The solvents are then pumped into the analytical column using a screw-driven syringe or reciprocating pumps.
2.2K