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A Uniform Shear Assay for Human Platelet and Cell Surface Receptors via Cone-plate Viscometry

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Platelet glycoprotein IIb. Chromosomal localization and tissue expression.

P F Bray¹, J P Rosa, G I Johnston

¹Department of Medicine, University of California, San Francisco 94143.

The Journal of Clinical Investigation

|December 1, 1987

Summary

Researchers identified the gene for GPIIb, a key platelet receptor, on chromosome 17. This finding confirms GPIIb

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Area of Science:

Molecular Biology
Cell Biology
Hematology

Background:

The GPIIb-IIIa complex is a crucial platelet surface receptor for cytoadhesive proteins.
Both GPIIb and GPIIIIa subunits are synthesized in human erythroleukemia (HEL) cells.

Purpose of the Study:

To isolate and characterize cDNA clones encoding the GPIIb subunit.
To determine the gene location and expression pattern of GPIIb.

Main Methods:

Screening of a HEL cell cDNA library using an oligonucleotide probe.
Nucleotide and amino acid sequencing of isolated cDNA clones.
Chromosome mapping using dual laser chromosome sorting.
Northern blot analysis for mRNA expression.

Main Results:

A cDNA clone encoding the carboxyl terminus of the GPIIb alpha subunit and the complete beta subunit was isolated and sequenced.
The GPIIb gene was mapped to chromosome 17.
A 3.4-kb GPIIb mRNA was detected in HEL cells.
GPIIb mRNA was not found in vascular endothelial cells or monocytes, suggesting cell-specific expression.

Conclusions:

The characterized cDNA confirms the structure of platelet GPIIb.
GPIIb is specifically expressed on platelet-megakaryocyte membranes.
GPIIb is distinct from alpha subunits of adhesion receptors in other tissues.