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Comparative Analysis of Serum Proteins Between Hepatitis B Virus Genotypes B and C Infection by DIA-Based
Yunqing Chen1,2, Dahai Wei1,2,3, Min Deng1,2,3
1Department of Infectious Diseases, Affiliated Hospital of Jiaxing University, Jiaxing, People's Republic of China.
Insights
Hepatitis B virus (HBV) genotypes B and C exhibit distinct serum protein profiles. This proteomic analysis identified potential biomarkers, VWF and C8B, differentiating between HBV genotypes.
Area of Science:
- Hepatology
- Proteomics
- Virology
Background:
- Hepatitis B virus (HBV) infection outcomes vary by genotype.
- Understanding molecular differences between HBV genotypes B and C is crucial for targeted therapies.
Purpose of the Study:
- To compare serum protein profiles between patients infected with HBV genotype B and genotype C.
- To identify potential protein biomarkers distinguishing between HBV genotypes.
Main Methods:
- Serum samples from 54 patients (18 per group: HBV genotype B, HBV genotype C, healthy controls) were analyzed.
- Data-independent acquisition (DIA)-based liquid chromatography-mass spectrometry was employed for proteomic analysis.
Main Results:
- 1010 proteins were identified, with 53 differentially expressed in HBV genotype B and 59 in HBV genotype C patients compared to controls.
- Von Willebrand Factor (VWF) and Complement C8 Beta (C8B) were identified as differentially expressed proteins between HBV genotypes B and C.
Conclusions:
- HBV genotypes B and C are associated with distinct molecular profiles.
- VWF and C8B show potential as biomarkers for differentiating HBV genotypes B and C.
- Findings provide a basis for further research into genotype-specific molecular mechanisms.
Purpose:
In clinical practice, the clinicopathological profiles and outcomes of patients infected with hepatitis B virus (HBV) are different between genotypes B and C. However, little is known about the potential mechanism and differences in specific biological pathways associated with the different genotype. This study aimed to compare the serum protein profile between patients infected with HBV genotype B and those infected with HBV genotype C.
Patients And Methods:
A total of 54 serum samples from patients with chronic HBV genotype B infection and those with chronic HBV genotype C infection, and healthy controls were used for the proteomic analysis (n = 18 samples in per group). Serum proteomic profiles were analyzed using data-independent acquisition (DIA)-based liquid chromatography-mass spectrometry to identify differentially expressed proteins (up- or downregulation of at least 1.5-fold) between serum samples from HBV patients infected with HBV genotype B and those infected with genotype C.
Results:
We identified 1010 proteins, 53 of which were differentially expressed between the serum samples of the healthy controls and those of HBV genotype B infected patients, and 59 that were differentially expressed between the samples of the healthy controls and those of HBV genotype C infected patients. Furthermore, our results indicated that two proteins identified as being differentially expressed (VWF and C8B) have potential as biomarkers for distinguishing genotype B infected HBV patients from those infected with genotype C.
Conclusion:
The results of our DIA-based quantitative proteomic analysis revealed that HBV genotypes B and C are associated with different molecular profiles and may provide fundamental information for further detailed investigations of the molecular mechanism underlying these differences.
