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Circulatory microRNA expression profile for coronary artery calcification in chronic kidney disease patients
Bhooma Vijayaraghavan1, Sridharan Jeyamohan2, Giri Padmanabhan1
1Kidney care, 10th B cross, Thillai Nagar, Tiruchirappalli-620018, Tamilnadu, India.
Insights
Thirteen microRNAs show altered expression in chronic kidney disease (CKD) patients with coronary artery calcification. This microRNA profiling may aid in diagnosing cardiovascular disease in CKD patients.
Area of Science:
- Nephrology
- Cardiology
- Molecular Biology
Background:
- Coronary artery disease (CAD) is a leading cause of death in end-stage renal disease (ESRD) patients.
- MicroRNA (miRNA) profiling offers a molecular diagnostic approach for diseases.
- Chronic kidney disease (CKD) patients have a high risk of developing CAD, particularly coronary artery calcification.
Purpose of the Study:
- To profile microRNA expression in CKD patients with coronary artery calcification.
- To identify specific microRNAs associated with coronary artery calcification in CKD.
Main Methods:
- Study included 200 CKD patients (stages 3-5, non-dialysis) and healthy controls.
- Coronary artery calcification assessed using 1024 multi-slice cardiac CT scans for calcium scoring (>100).
- Serum miRNA microarray analysis performed on samples from high-calcium CKD patients and controls.
Main Results:
- Seven CKD patients exhibited significant upregulation and downregulation of circulating miRNAs compared to controls.
- Upregulated miRNAs included: mir21, mir67, mir390, mir56, mir250, mir65, and mir13.
- Downregulated miRNAs included: mir235, mir256, mir226, mir207, mir255, and mir193.
- No significant difference in left ventricle function was observed.
Conclusions:
- Thirteen microRNAs are potentially involved in coronary artery calcification in CKD patients.
- These identified miRNAs could serve as biomarkers for CAD in CKD.
- Further research is warranted to validate these findings and explore therapeutic implications.
Background & Aim:
Coronary artery disease (CAD) is the primary cause of mortality in patients with end stage renal disease (ESRD). MicroRNA profiling is proven as a powerful tool in the diagnosis of any disease at the molecular level. Hence, the present study aimed to profile the microRNA expression for CAD especially coronary artery calcification in CKD patients.
Materials And Methods:
Two hundread patients with CKD stages 3 to 5 without dialysis and healthy controls were included in this study. All two hundred patients underwent 1024 multi sliceardiac computed tomography (CT) scan for calcium scoring. The calcium scoring more than 100 have been included in the study. We performed miRNA microarray analysis from serum samples of seven high calcium scored with CKD patients and one control patients.
Results:
Seven patients have observed circulating miRNAs has significantly upregulated and downregulated when compared with control patients. mir21, mir 67, mir 390, mir 56, mir 250, mir 65 and mir 13 were up regulated and mir235, mir256, mir226, mir207, mir255, mir193 were downregulated. There was no significant difference in left ventricle function.
Conclusion:
13 microRNAs play a potential role in coronary artery calcification in CKD patients.
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