Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Targeting SYK to alleviate MDSC-driven immunosuppression and augment anti-PD1 efficacy.

Science China. Life sciences·2026

Same author

Three-dimensionally printed mesoporous bioactive glass for craniomaxillofacial bone regeneration: Material evolution, functional mechanisms, and clinical translation.

Cell transplantation·2026

Same author

Restoring cartilage-subchondral bone metabolism with biphasic magnesium microspheres ameliorate early osteoarthritis.

Bioactive materials·2026

Same author

Biomaterial-Integrated Electroporation for Therapeutic Delivery: From Gene Editing to Tumor Ablation and Immune Modulation.

Small (Weinheim an der Bergstrasse, Germany)·2026

Same author

The neuro-skeletal crosstalk: Mechanisms, clinical implications, and smart material interventions.

Journal of orthopaedic translation·2026

Same author

Closed-loop iron chelate recycling via molecularly imprinted hydrogels suppresses ferroptosis.

Nature communications·2026

Same journal

A Programmed Drug-Loaded and Penetration-Delivery Functionalized Microneedle Patch for Synergistic Obesity Treatment.

Advanced healthcare materials·2026

Same journal

Antibacterial and Immunomodulatory Coatings for Orthopedic Metal Implants: Biological Rationale, Design Strategies, and Translational Challenges.

Advanced healthcare materials·2026

Same journal

Butyrylated PGAM5-Triggered and GSH-Responsive Cysteine Polymer Nanoparticles for CBL0137 Delivery to Enhance Necroptosis in Prostate Cancer.

Advanced healthcare materials·2026

Same journal

Dual-Modal Phototherapeutic Nanoagents Eradicating Drug-Resistant Bacteria via Multi-Pathway of Membrane Disruption, Oxidative Damage, and Energy Metabolism Interference.

Advanced healthcare materials·2026

Same journal

Smartphone-Enabled Point-of-Care Biosensing Platform With Self-Calibration for Rapid Matrix-Resistant Detection of Multiple AMI Biomarkers in Whole Blood.

Advanced healthcare materials·2026

Same journal

Multimetal-Doped Nanoenzymes Reprogram Macrophages for Immunotherapy of Gouty Arthritis.

Advanced healthcare materials·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Oct 12, 2025

Micropatterning and Assembly of 3D Microvessels

Micropatterning and Assembly of 3D Microvessels

Published on: September 9, 2016

Engineered Customizable Microvessels for Progressive Vascularization in Large Regenerative Implants.

Cuidi Li¹, Xiaoyu Han¹, Zhenjiang Ma²

¹Department of Orthopaedics, Shanghai Key Laboratory for Prevention and Treatment of Bone and Joint Diseases, Shanghai Institute of Traumatology and Orthopaedics, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, 197 Ruijin 2nd Road, Shanghai, 200025, P. R. China.

Advanced Healthcare Materials

|November 19, 2021

Summary

This summary is machine-generated.

Engineered customizable microvessels (ECMVs) sprout and form vascular networks, enabling rapid pre-vascularization for tissue regeneration. This technique supports both soft and hard tissue implants, promoting healing and integration with host vessels.

Keywords:

injectable implants microfluidics microvessels regeneration vascularization

More Related Videos

Isolation of Murine Adipose Tissue-derived Microvascular Fragments as Vascularization Units for Tissue Engineering

Isolation of Murine Adipose Tissue-derived Microvascular Fragments as Vascularization Units for Tissue Engineering

Published on: April 30, 2017

Stepwise Cell Seeding on Tessellated Scaffolds to Study Sprouting Blood Vessels

Stepwise Cell Seeding on Tessellated Scaffolds to Study Sprouting Blood Vessels

Published on: January 14, 2021

Related Experiment Videos

Last Updated: Oct 12, 2025

Micropatterning and Assembly of 3D Microvessels

Micropatterning and Assembly of 3D Microvessels

Published on: September 9, 2016

Isolation of Murine Adipose Tissue-derived Microvascular Fragments as Vascularization Units for Tissue Engineering

Isolation of Murine Adipose Tissue-derived Microvascular Fragments as Vascularization Units for Tissue Engineering

Published on: April 30, 2017

Stepwise Cell Seeding on Tessellated Scaffolds to Study Sprouting Blood Vessels

Stepwise Cell Seeding on Tessellated Scaffolds to Study Sprouting Blood Vessels

Published on: January 14, 2021

Area of Science:

Biomaterials Engineering
Regenerative Medicine
Vascular Biology

Background:

Natural microvessel angiogenesis inspires new biomaterials.
Current tissue regenerative implants often lack sufficient vascularization.
Pre-vascularization is crucial for the success of large tissue implants.

Purpose of the Study:

To develop engineered customizable microvessels (ECMVs) that mimic natural angiogenesis.
To enable rapid vascular network formation within regenerative implants.
To provide a versatile strategy for pre-vascularizing both soft and hard tissue implants.

Main Methods:

Utilizing a cellular coaxial microfluidic extrusion technique combined with microsurgery.
Employing sacrificial printing technology for implant shape and vascular density customization.
Integrating ECMVs into large bone repair implants via injection and suturing.

Main Results:

ECMVs demonstrated angiogenic sprouting and formation of daughter vascular networks in vitro.
In vivo studies confirmed vascularization promotion and anastomosis with host vessels.
Personalized microvessel networks were created using a 3D printer connected to the microfluidic system.

Conclusions:

ECMVs offer a straightforward engineering approach for rapid vascularization of large regenerative implants.
This technology facilitates the integration of implants with host tissues by promoting vascularization.
ECMVs hold significant potential for advancing soft and hard tissue regeneration therapies.