Dual Photoredox Catalysis Strategy for Enhanced Photopolymerization-Based Colorimetric Biodetection

Catalytic redox reactions have been employed to enhance colorimetric biodetection signals in point-of-care diagnostic tests, while their time-sensitive visual readouts may increase the risk of false results. To address this issue, we developed a dual photocatalyst signal amplification strategy that can be controlled by a fixed light dose, achieving time-independent colorimetric biodetection in paper-based tests. In this method, target-associated methylene blue (MB⁺) photocatalytically amplifies the concentration of eosin Y by oxidizing deactivated eosin Y (EYH^3-) under red light, followed by photopolymerization with eosin Y autocatalysis under green light to generate visible hydrogels. Using the insights from mechanistic studies on MB⁺-sensitized photo-oxidation of EYH^3-, we improved the photocatalytic efficiency of MB⁺ by suppressing its degradation. Lastly, we characterized 100- to 500-fold enhancement in sensitivity obtained from MB⁺-specific eosin Y amplification, highlighting the advantages of using dual photocatalyst signal amplification.