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Updated: Oct 12, 2025

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Multivariate analysis for organic milk authentication.

Siyan Xu1, Chaomin Zhao2, Xiaojun Deng2

  • 1School of Life Sciences, Shanghai University, Shanghai 200444, China; Technical Center for Animal, Plant and Food Inspection and Quarantine, Shanghai Customs, Shanghai 200135, China.

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
|November 19, 2021
PubMed
Summary
This summary is machine-generated.

A new model using stable isotopes, elements, and fatty acids can effectively differentiate organic milk from conventional milk. This approach provides a more robust method for verifying milk authenticity and quality.

Keywords:
Discriminant analysisMultivariate determinationNutritional qualityOrganic milkStable isotope ratios

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Area of Science:

  • Food Science
  • Analytical Chemistry
  • Biochemistry

Background:

  • Distinguishing organic milk (OM) from conventional milk (CM) is crucial for consumer trust and regulatory compliance.
  • Previous methods often rely on limited variables, potentially leading to less reliable models for milk authenticity testing.

Purpose of the Study:

  • To develop and validate a robust multivariate model for differentiating OM from CM.
  • To identify key discriminating parameters between organic and conventional milk sources.

Main Methods:

  • Construction of an orthogonal projection to latent structure-discriminant analysis (OPLS-DA) model.
  • Utilizing a comprehensive dataset including stable isotopes (δ13C, δ15N, δ18O), 51 elements, and 35 fatty acids (FAs).

Main Results:

  • The multivariate model demonstrated superior explanatory and predictive abilities (R2X = 0.647, R2Y = 0.962, Q2 = 0.821) compared to models with fewer variables.
  • Key discriminators identified include δ15N, Selenium (Se), δ13C, Europium (Eu), Potassium (K), and α-Linolenic acid (ALA).

Conclusions:

  • A multivariate approach integrating stable isotopes, elemental composition, and fatty acid profiles offers a powerful tool for identifying organic milk.
  • This method enhances the reliability of milk authenticity testing and can contribute to global databases for milk quality assessment.