iDHS-DT: Identifying DNase I hypersensitive sites by integrating DNA dinucleotide and trinucleotide information

DNase I hypersensitive sites (DHSs) is important for identifying the location of gene regulatory elements, such as promoters, enhancers, silencers, and so on. Thus, it is crucial for discriminating DHSs from non-DHSs. Although some traditional methods, such as Southern blots and DNase-seq technique, have the ability to identify DHSs, these approaches are time-consuming, laborious, and expensive. To address these issues, researchers paid their attention on computational approaches. Therefore, in this study, we developed a novel predictor called iDHS-DT to identify DHSs. In this predictor, the DNA sequences were firstly denoted by physicochemical properties (PC) of DNA dinucleotide and trinucleotide. Then, three different descriptors, including auto-covariance, cross-covariance, and discrete wavelet transform were used to collect related features from the PC matrix. Next, the least absolute shrinkage and selection operator (LASSO) algorithm was employed to remove these irrelevant and redundant features. Finally, these selected features were fed into support vector machine (SVM) for distinguishing DHSs from non-DHSs. The proposed method achieved 97.64% and 98.22% classification accuracy on dataset S₁ and S₂, respectively. Compared with the existing predictors, our proposed model has significantly improvement in classification performance. Experimental results demonstrated that the proposed method is powerful in identifying DHSs.