Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Mapping approaches to gene identification in humans.

R L White, J M Lalouel, G M Lathrop

    The Western Journal of Medicine
    |October 1, 1987
    PubMed
    Summary

    Genetic linkage mapping uses DNA markers to locate disease genes when biochemical defects are unknown. This approach aids in understanding genetic disorders and potential disease mechanisms.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Genomic analysis of Fas and FasL genes and absence of correlation with disease progression in AIDS.

    Immunogenetics·2004
    Same author

    Genomic analysis of Th1-Th2 cytokine genes in an AIDS cohort: identification of IL4 and IL10 haplotypes associated with the disease progression.

    Genes and immunity·2003
    Same author

    A genomewide scan for loci predisposing to type 2 diabetes in a U.K. population (the Diabetes UK Warren 2 Repository): analysis of 573 pedigrees provides independent replication of a susceptibility locus on chromosome 1q.

    American journal of human genetics·2001
    Same author

    Trans-ethnic fine mapping of a quantitative trait locus for circulating angiotensin I-converting enzyme (ACE).

    Human molecular genetics·2001
    Same author

    Associations of distinct variants of the intestinal mucin gene MUC3A with ulcerative colitis and Crohn's disease.

    Journal of human genetics·2001
    Same author

    A first high-density map of 981 biallelic markers on human chromosome 14.

    Genomics·2000

    Area of Science:

    • Human Genetics
    • Molecular Biology
    • Medical Genetics

    Background:

    • Most genetic defects are identified by phenotype, as the specific biochemical defect is often unknown.
    • Locating disease-causing genes typically relies on molecular methods, particularly genetic linkage analysis.
    • Genetic linkage involves tracking the coinheritance of a disease phenotype with specific DNA markers within families.

    Purpose of the Study:

    • To highlight the utility of genetic linkage mapping for identifying disease-associated genes.
    • To emphasize the importance of a dense human genetic map with numerous polymorphic markers.
    • To discuss the broader implications of gene mapping in understanding disease and molecular biology.

    Main Methods:

    • Utilizing coinheritance patterns of disease phenotypes and polymorphic DNA markers in affected families.

    Related Experiment Videos

  • Employing genetic linkage studies to map genes to specific chromosomal regions.
  • Developing and mapping a large number of polymorphic DNA markers in reference pedigrees.

    • Main Results:

    • Genetic linkage studies have successfully localized genes responsible for significant diseases, such as cystic fibrosis.
    • The development of a dense human genetic map with numerous polymorphic markers is crucial for efficient gene localization.
    • Ongoing efforts are identifying and mapping highly polymorphic DNA segments.

    Conclusions:

    • Genetic linkage mapping is a powerful tool for discovering disease genes, especially when biochemical pathways are uncharacterized.
    • A comprehensive genetic map facilitates the localization of any disease gene through linkage analysis.
    • Gene mapping advances our understanding of molecular biology, disease mechanisms, and early events in disorders like cancer.