Related Experiment Video
Updated: Oct 2, 2026

Spheroid Assay to Measure TGF-β-induced Invasion
Published on: November 16, 2011
Bone-derived and recombinant transforming growth factor beta's are potent inhibitors of tumor cell growth
J E Ranchalis1, L Gentry, Y Ogawa
1Oncogen, Seattle, WA 98121.
Abstract:
Two naturally occurring chrondogenesis inducing peptides have been purified to homogeneity from demineralized bovine bone. Cartilage-inducing factors A and B are the bone-derived equivalents of transforming growth factor-beta types I and II. Both peptides exhibit identical biological activities in chondrogenesis assays and stimulate anchorage independent cell growth. In this study we show that both bone-derived factors are potent (ng/ml) inhibitors of both DNA synthesis and the anchorage independent growth of a variety of human and non-human tumor cells. Unique in this study is also a comparison of the activities of these polypeptide growth factors with recombinant transforming growth factor type I expressed in mammalian cells.
Related Concept Videos
Inhibition of Cdk Activity
Mitogens and the Cell Cycle
Targeted Cancer Therapies
There are several types of targeted therapies against specific...
Regulation of Angiogenesis and Blood Supply
TGF - β Signaling Pathway
Inhibition of CDK Activity

