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Evaluation of OIDx Histoplasma Urinary Antigen EIA
Diego H Cáceres1,2, Beatriz L Gómez3, Ángela M Tobón4,5
1Centers for Disease Control and Prevention (CDC), 1600 Clifton Rd., Atlanta, GA, 30333, USA. diegocaceres84@gmail.com.
Abstract:
A sandwich enzyme immunoassay (EIA) for the detection of Histoplasma antigens (Ag) in urine, developed by Optimum Imaging Diagnostics (OIDx) was evaluated. A verification using a standardized reference panel of urine samples found sensitivity of 92%, specificity of 32% and accuracy of 51%. In this study, the OIDx Histoplasma urinary Ag EIA displayed high sensitivity, however, in non-histoplasmosis cases this EIA displayed false-positive results in 68% of specimens tested.
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