Identification of T Peripheral Helper (Tph) Cells
Vanessa S Wacleche1, Runci Wang1, Deepak A Rao2
1Division of Rheumatology, Inflammation, Immunology, Brigham and Women's Hospital and Harvard Medical School, Boston, MA, USA.
Methods in Molecular Biology (Clifton, N.J.)
|November 21, 2021
Summary
T peripheral helper (Tph) cells, crucial for B cell responses, are identified and distinguished from T follicular helper (Tfh) cells. These methods aid in exploring Tph cell roles in autoimmune diseases.
Area of Science:
- Immunology
- Cell Biology
Background:
- T peripheral helper (Tph) cells are CD4+ T cells that support B cell responses in tissues.
- Tph cells share some functions with T follicular helper (Tfh) cells but differ in molecular markers.
- Tph cells are implicated in the pathogenesis of autoimmune diseases.
Purpose of the Study:
- To provide detailed protocols for identifying and characterizing Tph cells.
- To distinguish Tph cells from Tfh cells using cytometry techniques.
- To facilitate research on the role of Tph cells in human diseases.
Main Methods:
- Flow cytometry and mass cytometry protocols are described.
- Identification of Tph cells based on PD-1, CXCR5, and CD4 expression.
- Characterization of Tph cell phenotype and function.
Main Results:
- Detailed methods for Tph cell identification are presented.
- Phenotypic and functional features of Tph cells are characterized.
- Distinction between Tph and Tfh cells is clarified.
Conclusions:
- Established protocols enable reliable identification of Tph cells.
- Understanding Tph cell biology is crucial for autoimmune disease research.
- These methods will advance the study of Tph cells in various human conditions.
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