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In Vivo Migration and Tfh Cell Interactions

Bo Liu1,2, Hai Qi3,4

  • 1Laboratory of Dynamic Immunobiology, Institute for Immunology, Beijing, China.

Methods in Molecular Biology (Clifton, N.J.)
|November 21, 2021
PubMed

Follicular T-helper cells play a key role in orchestrating the germinal center response. Cellular dynamics revealed by intravital imaging have helped deepen our understanding of how follicular T-helper cells develop and function in vivo. Here we describe routine methods to image T-cell dynamics during germinal center responses. Methods for quantitative analysis of imaging data are detailed using published examples.

Keywords:
Follicular T-helper cellsIntravital microscopyT-B interactionsTwo-photon imaging

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