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Therapeutic Modulation of T Follicular Helper Cells by Low-Dose IL-2 Treatment

Zhian Chen1, Kaili Liang2, Jun Deng2

  • 1The University of Queensland Diamantina Institute, Translational Research Institute, Brisbane, QLD, Australia.

Methods in Molecular Biology (Clifton, N.J.)
|November 21, 2021
PubMed

T follicular helper (Tfh) cells are a subset of specialized CD4+ T cell residing in B cell follicles and confer essential support for germinal center responses, which lead to the generation of long-lived humoral immunity. A great deal of evidence from the past 15 years indicate that excessive differentiation and dysregulated function of Tfh cells often promote autoimmunity by inducing autoantibody production. Interleukin-2 was identified as a major suppressor to inhibit Tfh differentiation. Therefore, IL-2 treatment was applied in suppressing Tfh function in mouse models and more recently in a clinical trial of patients with systemic lupus erythematosus. Here we describe a protocol for low-dose IL-2 treatment in a murine immunization model and on the assessment of the suppression of Tfh response using flow cytometry.

Keywords:
AutoimmunityFlow cytometryGerminal centerLow-dose IL-2 treatmentT follicular helper cells

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