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Interspecific Recombination Between Zucchini Tigre Mosaic Virus and Papaya Ringspot Virus Infecting Cucurbits in
Bin Peng1, Liming Liu1, Huijie Wu1
1Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences (CAAS), Zhengzhou, China.
Frontiers in Microbiology
|November 22, 2021
Summary
Interspecific recombination between papaya ringspot virus (PRSV) and zucchini tigre mosaic virus (ZTMV) was identified in China. This rare event offers insights into the evolution of these significant cucurbit viruses.
Area of Science:
- Plant Virology
- Molecular Evolution
- RNA Virus Genetics
Background:
- Recombination is a key driver of single-stranded RNA virus evolution, facilitating adaptation.
- While intraspecific recombination is common in potyviruses, interspecific recombination is rare.
- Papaya ringspot virus (PRSV) and zucchini tigre mosaic virus (ZTMV) are significant cucurbit pathogens within the Potyviridae family.
Purpose of the Study:
- To report and characterize a novel interspecific recombination event between PRSV and ZTMV.
- To investigate the prevalence and evolutionary implications of this interspecific recombination in cucurbit crops.
- To analyze the recombination site and its impact on virus characteristics.
Main Methods:
- Small RNA sequencing for recombinant detection in field samples.
- Overlapping RT-PCR for complete nucleotide sequence determination.
- Bioinformatic analyses including multiple sequence alignment, recombination detection, and phylogenetic analysis.
- Experimental characterization of recombinant isolates using cDNA infectious clones.
Main Results:
- Detection of PRSV-ZTMV interspecific recombinants in cucurbit samples from China.
- Confirmation of interspecific recombination with the recombination site located in the HC-pro region.
- Identification of an additional intraspecific recombination event within ZTMV populations.
- Experimental characterization of two recombinant isolates (KF8 and CX1) regarding symptoms and host range.
- Incidence rates of 16% and 19.4% for the interspecific recombinant virus in Henan province during 2017 and 2018 surveys.
Conclusions:
- The study confirms a rare interspecific recombination event between PRSV and ZTMV in cucurbits.
- The novel recombination pattern within the HC-pro gene provides new insights into potyvirus evolution.
- Findings contribute to understanding the prevalence, evolution, and adaptation of ZTMV and PRSV in agricultural settings.
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