Related Experiment Video

Updated: Oct 12, 2025

In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice
07:02

In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice

Published on: August 23, 2019

7.5K

MiR-186 Suppressed Growth, Migration, and Invasion of Lung Adenocarcinoma Cells via Targeting Dicer1

Juan Wang1, Yi Zhang2, Fanghong Ge3

  • 1Department of Oncology, Tongzhou Hospital Affiliated to Nantong University, Nantong, Jiangsu 226300, China.

Journal of Oncology
|November 22, 2021
PubMed
Abstract

Insights

MicroRNA-186 (miR-186) is downregulated in lung adenocarcinoma (LUAD). Restoring miR-186 inhibits LUAD cell proliferation, migration, and invasion by targeting Dicer1, suggesting a critical regulatory role.

Area of Science:

  • Oncology
  • Molecular Biology
  • Gene Regulation

Background:

  • Non-small-cell lung cancer (NSCLC), particularly lung adenocarcinoma (LUAD), is a leading cause of cancer mortality worldwide.
  • MicroRNAs (miRNAs) are crucial regulators of gene expression involved in cancer development and progression.
  • The specific role of miR-186 in LUAD pathogenesis requires further investigation.

Purpose of the Study:

  • To investigate the biological functions of miR-186 in lung adenocarcinoma (LUAD).
  • To elucidate the molecular mechanisms underlying miR-186's role in LUAD progression.

Main Methods:

  • Analysis of miR-186 expression in LUAD tissues and cell lines using immunohistochemistry, RT-PCR, and western blot.
  • Assessment of cell proliferation, migration, and invasion using CCK-8 and Transwell assays.
  • Luciferase assays to confirm the direct targeting of Dicer1 by miR-186 and functional rescue experiments.

Main Results:

  • Lower miR-186 expression was observed in LUAD tissues, correlating with advanced TNM stage and lymph node metastasis.
  • Upregulation of miR-186 significantly suppressed LUAD cell proliferation, migration, and invasion.
  • Dicer1 was identified as a direct target of miR-186, and its overexpression reversed the inhibitory effects of miR-186.

Conclusions:

  • The miR-186/Dicer1 pathway plays a critical role in the regulation of LUAD cell proliferation, migration, and invasion.
  • miR-186 functions as a tumor suppressor in LUAD.
  • Targeting the miR-186/Dicer1 pathway may offer a potential therapeutic strategy for LUAD.

More Related Videos

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis11:44

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Published on: March 30, 2019

7.7K
An In Vitro Protocol for Evaluating MicroRNA Levels, Functions, and Associated Target Genes in Tumor Cells09:45

An In Vitro Protocol for Evaluating MicroRNA Levels, Functions, and Associated Target Genes in Tumor Cells

Published on: May 21, 2019

8.5K

Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.2K
Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
6.4K
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.7K
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
26.7K
siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
17.2K