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Related Concept Videos

Precipitation Titration: Endpoint Detection Methods01:19

Precipitation Titration: Endpoint Detection Methods

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In argentometric precipitation titrations, endpoints can be detected visually by the Mohr, Volhard, and Fajans methods. In the Mohr method, adding a soluble chromate indicator gives an initial yellow color to the analyte solution. As the titrant is added, the first excess of silver ions forms a red silver chromate precipitate, marking the endpoint. The solution pH should be maintained at about 8 by adding solid CaCO3.
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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
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Related Experiment Video

Updated: Oct 12, 2025

Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds
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Starch-Based Carbon Dots for Nitrite and Sulfite Detection.

Panyong Wang1, Yan Zhang2, Yulu Liu1,3

  • 1School of Biomedical Engineering (Suzhou), Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, China.

Frontiers in Chemistry
|November 22, 2021
PubMed
Summary

Researchers developed a novel "on-off-on" fluorescent probe using carbon dots from starch. This method efficiently detects nitrite and sulfite concentrations, crucial for health and environmental monitoring, and enables intracellular bioimaging.

Keywords:
carbon dotsfluorescence detectionnitrite and sulfiteon-off-onstarch

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Area of Science:

  • Environmental Science
  • Analytical Chemistry
  • Biotechnology

Background:

  • Nitrite (NO2-) and sulfite (SO32-) are vital analytes in environmental and health studies.
  • Existing methods for detecting these ions can be complex or lack efficiency.
  • There is a need for facile, sensitive, and green analytical techniques.

Purpose of the Study:

  • To synthesize a multi-functional fluorescent probe for detecting nitrite and sulfite.
  • To develop an efficient and green method for quantifying NO2- and SO32- concentrations.
  • To explore the application of the probe in intracellular bioimaging.

Main Methods:

  • Synthesis of carbon dots (CDs) using starch as the sole raw material.
  • Utilizing the fluorescence 'on-off-on' response of CDs to NO2- and SO32-.
  • Investigating fluorescence quenching by nitrite (charge transfer) and recovery by sulfite (redox reaction).

Main Results:

  • The synthesized CDs exhibited an 'on-off-on' fluorescence response to sequential addition of nitrite and sulfite.
  • Achieved low limits of detection: 0.425 μM for nitrite and 0.243 μM for sulfite.
  • Demonstrated successful intracellular bioimaging of NO2- and SO32- due to low cytotoxicity and good analytical performance.

Conclusions:

  • The starch-derived carbon dots offer a facile, efficient, and green fluorescent probe for simultaneous nitrite and sulfite detection.
  • The unique 'on-off-on' response mechanism provides a novel approach for ion analysis.
  • The probe's effectiveness in intracellular bioimaging highlights its potential for biological and environmental applications.