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Related Experiment Videos

Clathrin heavy chain: molecular cloning and complete primary structure.

T Kirchhausen1, S C Harrison, E P Chow

  • 1Department of Anatomy and Cellular Biology, Harvard Medical School, Boston, MA 02115.

Proceedings of the National Academy of Sciences of the United States of America
|December 1, 1987
PubMed
Summary

Researchers sequenced the rat clathrin heavy chain, revealing its structure and function. This protein is crucial for cellular processes and highly conserved across mammals.

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Area of Science:

  • Molecular Biology
  • Cell Biology
  • Protein Structure

Background:

  • Clathrin is a key protein complex involved in intracellular trafficking.
  • Understanding the heavy chain's structure is essential for elucidating clathrin-mediated endocytosis.
  • Previous studies have provided partial information on clathrin heavy chain structure.

Purpose of the Study:

  • To determine the complete amino acid sequence of the rat clathrin heavy chain.
  • To identify structural landmarks and functional domains within the heavy chain.
  • To investigate potential sites of interaction with clathrin light chains.

Main Methods:

  • Deduction of the amino acid sequence from cDNA clones.
  • Bioinformatic analysis to predict protein properties (e.g., molecular weight).

Related Experiment Videos

  • Comparative sequence analysis with other mammalian clathrin heavy chains.

    • Main Results:

    • The 1675-amino acid sequence of rat clathrin heavy chain was deduced, predicting a Mr 191,569 protein.
    • Polarity was established, with the terminal domain at the amino terminus and an unusual carboxyl terminus sequence.
    • A potential heavy-chain-light-chain interaction site was identified in the proximal segment.

    Conclusions:

    • The primary structure of the rat clathrin heavy chain is highly conserved among mammals.
    • The deduced sequence provides a detailed map of structural landmarks and potential functional regions.
    • The findings contribute to a deeper understanding of clathrin assembly and function in cellular processes.