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Updated: Oct 12, 2025
Derivation of Mouse Trophoblast Stem Cells from Blastocysts
Published on: June 8, 2010
Rapid generation of murine haploid-induced trophoblast stem cells via a Tet-on system
Mei Xu1, Wenhao Zhang1, Mengyang Geng1
1State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy, Tianjin Central Hospital of Gynecology Obstetrics/Tianjin Key Laboratory of Human Development and Reproductive Regulation, Nankai University, Tianjin 300350, China.
Abstract:
Haploid trophoblast stem cells (TSCs) are advanced in studying placental development for their placental precursor and homozygous features. Here, we describe how to generate haploid-induced TSCs (haiTSCs) from haploid embryonic stem cells with a Tet-on system. Our haiTSCs can maintain haploidy long-term and can produce genome-wide mutants combined with transposons. It is promising in high-throughput genetic screening of trophoblast-specific modulators. For complete details on the use and execution of this protocol, please refer to Peng et al. (2019).
