Circulating miR-27b as a Biomarker of the Development and Progression of Carotid Artery Stenosis
Tingting Lu1, Xin Li1, Chunxi Long2
1Encephalopathy Rehabilitation Department, Weifang Hospital of Traditional Chinese Medicine, Weifang, China.
Objective:
A close relationship of microRNAs (miRNAs) with various human diseases has been widely reported, including cardiovascular disease. The current study attempted to examine the abnormal expression of miR-27b in asymptomatic carotid artery stenosis (ACAS), its diagnostic value and predictive value for the development of ACAS were also assessed.
Methods:
Clinical serum samples were collected from both ACAS patients and healthy individuals, and levels of miR-27b in the clinical samples were detected using Real-time quantitative PCR. Cerebral ischemia events (CIEs) of patients during the 5-year follow-up were collected. The diagnostic and predictive values of serum miR-27b was assessed via plotting Receiver operating characteristic (ROC) and Kaplan-Meier curves. Multivariate cox regression analysis was performed for clinical independent index analysis.
Results:
ACAS patients had higher levels of miR-27b than the healthy subjects. There were close association of serum miR-27b levels with total cholesterol (TC) level, absence of hypertension and degree of carotid stenosis. High levels of miR-27b could differentiate ACAS cases from healthy subjects, and predicted the high incidence of CIEs. MiR-27b could be used as an independent predictor of cerebrovascular events via multiple Cox regression analysis (P = .031).
Conclusion:
The high level of miR-27b can predict the occurrence of ACAS, and is closely related to the subsequent occurrence of CIEs. The present results provide evidence for circulating miR-27b as a diagnostic and prognostic marker in patients with ACAS.
Insights
High levels of miR-27b indicate asymptomatic carotid artery stenosis (ACAS) and predict future cerebral ischemia events (CIEs). Circulating miR-27b serves as a valuable diagnostic and prognostic marker for ACAS patients.
Area of Science:
- Biochemistry
- Genetics
- Cardiovascular Medicine
Background:
- MicroRNAs (miRNAs) are implicated in various human diseases, including cardiovascular conditions.
- Asymptomatic carotid artery stenosis (ACAS) is a significant risk factor for cerebrovascular events.
- Understanding the role of specific miRNAs in ACAS is crucial for early diagnosis and risk prediction.
Purpose of the Study:
- To investigate the expression levels of miR-27b in patients with ACAS.
- To evaluate the diagnostic and predictive potential of serum miR-27b for ACAS and subsequent cerebral ischemia events (CIEs).
Main Methods:
- Serum samples from ACAS patients and healthy controls were analyzed using Real-time quantitative PCR to determine miR-27b levels.
- Patient follow-up data for 5 years were collected to record the incidence of CIEs.
- Receiver operating characteristic (ROC) curves, Kaplan-Meier analysis, and multivariate Cox regression were employed to assess diagnostic and predictive values.
Main Results:
- Serum miR-27b levels were significantly higher in ACAS patients compared to healthy individuals.
- Elevated miR-27b levels correlated with total cholesterol, absence of hypertension, and the degree of carotid stenosis.
- High miR-27b levels effectively differentiated ACAS cases and predicted a higher incidence of CIEs, serving as an independent predictor (P=.031).
Conclusions:
- Elevated serum miR-27b is a potential biomarker for predicting ACAS occurrence.
- Circulating miR-27b levels are closely associated with the subsequent risk of CIEs in ACAS patients.
- These findings support the utility of miR-27b as a diagnostic and prognostic marker for ACAS.
