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Prolonged refrigeration does not alter isoprostanes concentration in human milk
Chiara Peila1, Mariangela Longini2,3, Anna Laura Toni2
1Neonatology Unit, Department of Public Health and Pediatrics, University of Turin, Torino, Italy.
Summary
Human milk stored at 4°C for up to 96 hours retains its quality. This study found no significant changes in key oxidative stress markers, ensuring nutritional integrity and bioactivity.
Area of Science:
- Biochemistry
- Human Milk Research
- Nutritional Science
Background:
- The Academy of Breastfeeding Medicine recommends refrigerating human milk at 4°C for up to 4 days.
- Limited research exists on how milk composition changes during this recommended refrigeration period.
Purpose of the Study:
- To investigate the impact of refrigerating human milk at 4°C for up to 96 hours on specific oxidative stress markers.
- To analyze changes in F2-isoprostanes, F4-neuroprostanes, and F3-isoprostanes during cold storage.
Main Methods:
- Human milk samples from 9 healthy mothers were collected and stored at 4°C for 0, 24, 48, 72, and 96 hours.
- Samples were immediately frozen at -80°C after storage periods for analysis.
- Isoprostane concentrations were determined using Liquid Chromatography-Mass Spectrometry and Liquid Chromatography-Tandem Mass Spectrometry.
Main Results:
- Isoprostanes (F2-, F4-neuro-, and F3-isoprostanes) were detected in all human milk samples.
- No statistically significant changes in the concentration of these isoprostanes were observed over the 96-hour storage period.
Conclusions:
- This study confirms the presence of F2-isoprostanes, F4-neuroprostanes, and F3-isoprostanes in human milk.
- Refrigeration of human milk at 4°C for up to 96 hours does not significantly alter its bioactivity or nutritional quality concerning these biomarkers.
