Fast-iterative automatic reconstruction method for quantitative phase image with reduced phase perturbations in...

Area of Science:

• Optical Engineering
• Microscopy
• Image Reconstruction

Background:

• Off-axis digital holographic microscopy (DHM) is a powerful technique for quantitative phase imaging.
• Accurate reconstruction of complex object information in DHM is often challenged by phase perturbations.
• Existing methods may suffer from slow processing or inaccuracies in compensating for wave tilt.

Purpose of the Study:

• To develop an automatic and fast reconstruction algorithm for off-axis DHM.
• To precisely compensate for phase perturbations caused by the interference angle between reference and object waves.
• To improve the accuracy and efficiency of complex object information recovery in DHM.

Main Methods:

• A novel reconstruction algorithm minimizing a cost function to find the optimal numerical conjugated reference beam.
• Automatic estimation of the interference angle between object and reference waves.
• Validation using a manufactured phase target and biological samples.

Main Results:

• Successfully reconstructed complex object information with phase images free from perturbations.
• Achieved precise estimation of the interference angle between object and reference waves.
• Demonstrated significantly reduced processing time compared to conventional methods.

Conclusions:

• The proposed algorithm offers a precise and efficient solution for complex object reconstruction in off-axis DHM.
• This method enhances the quality of reconstructed phase images by eliminating undesired phase perturbations.
• The validated performance on diverse samples highlights its potential for various scientific applications.