Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

DNA Isolation01:24

DNA Isolation

42.2K
DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...
42.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

DNA metabarcoding to estimate diet overlap between the introduced Joro spider (Trichonephila clavata) and three native orb-weaving spiders.

PloS one·2026
Same author

Life-stage specific predatory efficiency of 2 generalist predators of Bemisia tabaci (Hemiptera: Aleyrodidae).

Journal of economic entomology·2026
Same author

Psychometric properties and validation of short health literacy screening (SHLS) to identify low health literacy.

Health promotion perspectives·2026
Same author

Feasibility of preoperative patient self-assessed frailty: a single-centre pilot study.

BJA open·2026
Same author

Willingness to Pay for a Service Package Solving the Problems of Patients With Cleft Lip and Palate Referred to the Cleft Lip and Palate Center.

International journal of paediatric dentistry·2025
Same author

Leaf trichome and insecticide interactions relative to Bemisia tabaci (Hemiptera: Aleyrodidae) management in a cotton-cucurbit strip cropping arrangement.

Journal of economic entomology·2025

Related Experiment Video

Updated: Oct 12, 2025

Laboratory Protocol for Genetic Gut Content Analyses of Aquatic Macroinvertebrates Using Group-specific rDNA Primers
10:17

Laboratory Protocol for Genetic Gut Content Analyses of Aquatic Macroinvertebrates Using Group-specific rDNA Primers

Published on: October 5, 2017

9.0K

Rapid PCR-based method for herbivore dietary evaluation using plant-specific primers.

Arash Kheirodin1, Mohammad Sayari2, Jason M Schmidt1

  • 1Department of Entomology, University of Georgia, Tifton, GA, United States of America.

Plos One
|November 22, 2021
PubMed
Summary

Researchers developed new plant-specific DNA primers to rapidly identify crops consumed by polyphagous pests. This cost-effective method aids in understanding pest diets and improving agricultural pest management strategies.

More Related Videos

Genotyping of Plant and Animal Samples without Prior DNA Purification
11:00

Genotyping of Plant and Animal Samples without Prior DNA Purification

Published on: September 24, 2012

31.4K
Assessment of DNA Contamination in RNA Samples Based on Ribosomal DNA
13:16

Assessment of DNA Contamination in RNA Samples Based on Ribosomal DNA

Published on: January 22, 2018

21.7K

Related Experiment Videos

Last Updated: Oct 12, 2025

Laboratory Protocol for Genetic Gut Content Analyses of Aquatic Macroinvertebrates Using Group-specific rDNA Primers
10:17

Laboratory Protocol for Genetic Gut Content Analyses of Aquatic Macroinvertebrates Using Group-specific rDNA Primers

Published on: October 5, 2017

9.0K
Genotyping of Plant and Animal Samples without Prior DNA Purification
11:00

Genotyping of Plant and Animal Samples without Prior DNA Purification

Published on: September 24, 2012

31.4K
Assessment of DNA Contamination in RNA Samples Based on Ribosomal DNA
13:16

Assessment of DNA Contamination in RNA Samples Based on Ribosomal DNA

Published on: January 22, 2018

21.7K

Area of Science:

  • Agricultural Entomology
  • Molecular Ecology
  • Plant Genomics

Background:

  • Polyphagous pests cause significant global economic losses in agriculture.
  • Current methods for assessing pest diets are time-consuming and labor-intensive.
  • Molecular gut content analysis, while effective, is costly and slow.

Purpose of the Study:

  • To develop a rapid, specific, sensitive, and cost-effective method for evaluating the diets of polyphagous pests.
  • To design and validate plant-specific primers for identifying crop species in pest guts.
  • To facilitate a better understanding of pest feeding habits in agroecosystems.

Main Methods:

  • Designed plant-specific primers targeting specific genes (VE-1, PR-1-5) from whole plant genome sequences.
  • Developed two multiplex polymerase chain reaction (PCR) primer mixes capable of identifying multiple crop species simultaneously.
  • Validated primer specificity and sensitivity for 14 important crop species.

Main Results:

  • Successfully designed species-specific primers for 14 key crop species.
  • Developed two multiplex primer mixes, one identifying nine species and the other five species per PCR reaction.
  • Achieved amplicon sizes ranging from 115 to 407 bp, suitable for rapid analysis.

Conclusions:

  • The developed primer mixes offer a rapid, sensitive, and specific tool for polyphagous pest dietary evaluation.
  • This method will accelerate research into pest diet preferences and crop consumption ranges.
  • Findings will support the development of improved crop rotation strategies to mitigate pest damage.